Nonconservative integration and diversity of a new family of integrative and conjugative elements associated with antibiotic resistance in zoonotic pathogen Streptococcus suis
Nonconservative integration and diversity of a new family of integrative and conjugative elements associated with antibiotic resistance in zoonotic pathogen Streptococcus suis
复制标题
与人畜共患病原体猪链球菌抗生素耐药性相关的新整合和共轭元件家族的非保守整合和多样性
DOI：
10.1016/j.vetmic.2021.109009
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发表时间：
2021-02-25
影响因子：
3.3
通讯作者：
Wang, Liping
中科院分区：
文献类型：
--
作者：
Yi, Sida;Huang, Jinhu;Wang, Liping
链球菌对大环内酯类和四环素类抗生素的耐药主要是由于获得了ICESa 2603家族的整合和接合元件（ICE），这些元件携带有β（B）和泰特（O）。但关于ICE与三联体丝氨酸整合酶的转移性及其生理后果的研究尚不多见。本研究检测了ICESsuYZDH1_SSU0877的可转移性，并评估了ICE转移和整合到受体中后的生理后果。ICESsuYZDH1_SSU0877是一种新型的具有三重丝氨酸整合酶的ICESa 2603家族样ICE，其携带有三重丝氨酸整合酶，并且是一种新型的具有三重丝氨酸整合酶的双（B）和泰特（O）的ICESa 2603家族样ICE。ICESsuYZDH 1样ICE在S.根据GenBank中1334条猪流感病毒基因组序列，对我国330株猪流感病毒进行了基因组序列分析。ICESsuYZDH 1在SSU 1797基因中的非保守性转移是通过在SSU 0877位点外整合ICESsuYZDH 1而实现的。2-/4-nt（5'-TC-3'/5'-TCCC-3？）在SSU 0877和SSU 1797位点分别观察到（5 '-GC-3'/5 '-TCCC-3'）。接合子的适应度成本较低，生长缓慢，与受体菌株的竞争较少。连续传代表明ICESsuYZDH 1能够持续存在并保持稳定的抗性表型。对来自公共基因组数据库和我们的临床分离株的ICESsuYZDH 1样ICE的综合分析揭示了通过在SSU 0877、SSU 0468、SSU 1262和SSU 1797位点整合的ICE的广泛性和多样性。ICESsuYZDH 1-like ICE可以稳定地共存于宿主染色体上的多个附着位点，这可能是由三联体丝氨酸整合酶介导的。ICESsuYZDH 1家族的非保守整合和多样性可能有助于ICE的进化以及大环内酯类和四环素耐药在S.猪。
Macrolide and tetracycline resistance in streptococci is mainly caused by acquisition of integrative and conjugative elements (ICEs) of the ICESa2603 family carrying erm(B) and tet(O). But the characteristics about the transferability and physiological consequences of ICEs with triplet serine integrases are still rare. This study tested the transferability of ICESsuYZDH1_SSU0877, a novel erm(B)-and tet(O)-carrying ICESa2603 family-like ICE with triplet serine integrases, and evaluated the physiological consequences after ICE transferred and integrated into recipient. The prevalence of ICESsuYZDH1-like ICEs in S. suis was analyzed based on 1334 genomic sequences available in GenBank and examined in 330 clinical isolates in China. Nonconservative transfer was observed by integrating of ICESsuYZDH1 into SSU1797 gene besides the primary SSU0877 site. Imperfect direct repeats of 2-/4-nt (5'-TC-3'/5'-TCCC-3?) and (5'-GC-3'/5'-TCCC-3') were observed at SSU0877 and SSU1797 sites, respectively. The transconjugant suffered a weak fitness cost with stunted growth and less competition with recipient strain. Successive passages indicate the ICESsuYZDH1 could be persist and endued stable resistant phenotype. Comprehensive analysis of the ICESsuYZDH1-like ICEs from both public genome database and our clinical isolates revealed the widespread and diversity of the ICEs by integration at the sites of SSU0877, SSU0468, SSU1262, and SSU1797. The ICESsuYZDH1-like ICEs could stably co-exist within the host chromosome at more than one attachment sites, which is probably mediated by the triplet serine integrases. Nonconservative integration and diversity of the ICESsuYZDH1 family of ICEs might have contributed to the evolution of ICEs and the dissemination of macrolide and tetracycline resistance in S. suis.