Molecular cytogenetics and phylogenetic analysis of Brazilian leaf frog species of the genera Phyllomedusa and Phasmahyla (Hylidae: Phyllomedusinae)
Molecular cytogenetics and phylogenetic analysis of Brazilian leaf frog species of the genera Phyllomedusa and Phasmahyla (Hylidae: Phyllomedusinae)
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DOI：
10.1139/cjz-2013-0301
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发表时间：
2014-09-01
影响因子：
1.4
通讯作者：
Costa, Marco Antonio
中科院分区：
文献类型：
--
作者：
Barth, Adriane;Vences, Miguel;Costa, Marco Antonio
在这项研究中，我们描述了叶蛙Phyllomedusa bahiana Lutz，1925，Phyllomedusa burmeisteri Boulenger，1882，Phyllomedusa nordestina Caramaschi，2006，Phyllomedusa rohdei Mertens，1926，Phyllomedusa hypocalalis（Daudin，1800），Phasmahyla tubabilis Cruz，Feio和Nascimento，2008的核型和分子系统发育关系。我们分析了核型C-显带，荧光染料染色，荧光原位杂交端粒探针，并推断遗传学利用核酪氨酸酶和线粒体16 S rDNA序列。北方拟步行虫的异染色质分布与其他物种不同，巴希拟步行虫和罗氏拟步行虫具有明显的间质端粒序列。分子分析证实了目前的分类学分类，每个物种的线粒体DNA序列分组，通常没有单倍型共享的核基因。我们还将P. burmeisteri的分布范围扩展到北方巴伊亚州，并将P. bahiana限制在巴伊亚州南部，P. bahiana的分布范围离散地扩展到米纳斯吉拉斯州的北部和东北部。酪氨酸酶单倍型网络显示来自巴伊亚的波尔图塞古罗的巴伊亚原杆菌和伯氏原杆菌之间共享单倍型。在此基础上，我们提出了一个新的分布在这两个物种之间的重叠区域，根据我们的数据范围之间的波尔图塞古罗和卡马坎，在巴伊亚州。
In this study we describe the karyotypes and molecular phylogenetic relationships of the leaf frogs Phyllomedusa bahiana Lutz, 1925, Phyllomedusa burmeisteri Boulenger, 1882, Phyllomedusa nordestina Caramaschi, 2006, Phyllomedusa rohdei Mertens, 1926, Phyllomedusa hypochondrialis (Daudin, 1800), and Phasmahyla spectabilis Cruz, Feio and Nascimento, 2008. We analyzed the karyotypes using C-banding, fluorochrome staining, and fluorescence in situ hybridization of telomeric probe, and inferred phylogeny using nuclear tyrosinase and mitochondrial 16S rDNA sequences. Heterochromatin distribution in P. nordestina diverged from the other species, and P. bahiana and P. rohdei showed evident interstitial telomere sequences. Molecular analyzes confirmed the current taxonomic classification, grouping mitochondrial DNA sequences of each species, and usually without haplotype sharing in the nuclear gene. We also extended the distribution of P. burmeisteri to northern Bahia state and restricted P. bahiana to southern Bahia state, with a discrete extension of the range of P. bahiana to the north and northeast of Minas Gerais state. The tyrosinase haplotype network showed a haplotype shared between P. bahiana and P. burmeisteri from Porto Seguro, Bahia. Based on this, we suggest a new distribution in the overlapping area between these two species, which according to our data ranges between Porto Seguro and Camacan, in Bahia state.