The Expression of Melanoma-Associated Antigen D2 Both in Surgically Resected and Serum Samples Serves as Clinically Relevant Biomarker of Gastric Cancer Progression
The Expression of Melanoma-Associated Antigen D2 Both in Surgically Resected and Serum Samples Serves as Clinically Relevant Biomarker of Gastric Cancer Progression
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DOI：
10.1245/s10434-015-4457-8
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发表时间：
2016-02-01
影响因子：
3.7
通讯作者：
Kodera, Yasuhiro
中科院分区：
文献类型：
--
作者：
Kanda, Mitsuro;Nomoto, Shuji;Kodera, Yasuhiro
敏感的生物标志物是必要的风险分类胃癌（GC）患者，特别是那些在远处转移的风险。黑色素瘤相关抗原（法师）-D2已被报道在大肠癌中的细胞粘附和肿瘤细胞转移潜能的过程中发挥作用。本研究旨在寻找一种新的胃癌临床相关生物标志物，从mRNA和蛋白水平分析MAGE-D2在胃癌细胞系和临床标本（手术标本和血清）中的表达。MAGE-D2 mRNA在胃癌组织中的表达水平与癌旁正常组织相似，与胃癌分化程度及亚型无关。胃癌组织中MAGE-D2 mRNA表达水平高于癌旁正常组织的有101例（45%）。胃癌组织中MAGE-D2 mRNA表达增高与远处转移和早期复发相关，是独立的预后因素（危险比2.27，95%可信区间1.39-3.74，P = 0.001）。血清MAGE-D2水平从健康志愿者到局限性GC患者，然后到扩展性GC患者（IV期）呈逐步增加。术前血清MAGE-D2水平> 130 pg/ml的胃癌患者预后较低，MAGE-D2与胃癌的转移潜能有关，可能是胃癌恶性行为的一个有前景的生物标志物。
Sensitive biomarkers are necessary for risk classification of patients with gastric cancer (GC), especially ones at risk of distant metastases. Melanoma-associated antigen (MAGE)-D2 has been reported to play a role in the process of cell adhesion and metastatic potential of tumor cells in colorectal cancer. The purpose of this study was to identify a novel clinically relevant biomarker of GC.Expression analysis of MAGE-D2 was conducted in GC cell lines and clinical samples (surgical specimen and serum) in both mRNA and protein level. Correlations between MAGE-D2 expression status and clinicopathological factors were evaluated.MAGE-D2 mRNA expression levels were similar between GC tissues and the corresponding normal adjacent tissues and were independent of GC differentiation or subtype. In 101 (45 %) of 225 patients, the expression level of MAGE-D2 mRNA was increased in GC tissues compared with the corresponding normal adjacent tissues. Increased expression of MAGE-D2 mRNA in GC tissues was associated with distant metastasis and early recurrence and was an independent prognostic factor (hazard ratio 2.27, 95 % confidence interval 1.39-3.74, P = 0.001). There was a stepwise increase in serum MAGE-D2 level going from healthy volunteers to patients with localized GC and then to those with extended GC (stage IV). Patients with preoperative serum MAGE-D2 levels > 130 pg/ml had a more unfavorable prognosis than those with levels a parts per thousand currency sign130 pg/ml.MAGE-D2 was associated with metastatic potential of GC and may represent a promising biomarker, both in gastric tissues and serum samples, for malignant behavior of GC.