Fabrication of complex three-dimensional tissue architectures using a magnetic force-based cell patterning technique
Fabrication of complex three-dimensional tissue architectures using a magnetic force-based cell patterning technique
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DOI：
10.1007/s10544-009-9284-x
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发表时间：
2009-08-01
影响因子：
2.8
通讯作者：
Kamihira, Masamichi
中科院分区：
文献类型：
--
作者：
Akiyama, Hirokazu;Ito, Akira;Kamihira, Masamichi
我们描述了使用基于磁力的组织工程技术制造三维组织结构，其中细胞组织是由磁力控制的。用磁性阳离子脂质体(MCL)标记靶细胞，从而可以通过施加磁场来操纵MCL标记的细胞。利用磁聚集器在单层细胞或皮肤组织上成功构建了标记MCLS的人脐静脉内皮细胞(HUVECs)的线状图案化。在均匀的磁力作用下，通过积累MCL标记的细胞，也可以在培养表面诱导多层细胞片。基于这些结果，我们试图构建复杂的多层成肌细胞C2C12细胞片。在此，通过交替C2C12细胞的磁积聚形成细胞层的过程和HUVECs在细胞层上的磁性图案化的过程来嵌入图案化的HUVECs。该技术可能适用于组织工程所需的复杂组织结构的制造。
We describe the fabrication of three-dimensional tissue constructs using a magnetic force-based tissue engineering technique, in which cellular organization is controlled by magnetic force. Target cells were labeled with magnetite cationic liposomes (MCLs) so that the MCL-labeled cells could be manipulated by applying a magnetic field. Line patterning of human umbilical vein endothelial cells (HUVECs) labeled with MCLs was successfully created on monolayer cells or skin tissues using a magnetic concentrator device. Multilayered cell sheets were also inducible on a culture surface by accumulating MCL-labeled cells under a uniform magnetic force. Based on these results, we attempted to construct a complex multilayered myoblast C2C12 cell sheet. Here, patterned HUVECs were embedded by alternating the processes of magnetic accumulation of C2C12 cells for cell layer formation and magnetic patterning of HUVECs on the cell layers. This technique may be applicable for the fabrication of complex tissue architectures required in tissue engineering.