Reprogramming of Oncogene Expression in Gingival Mesenchymal Stem Cells Following Long-Term Culture In Vitro
Reprogramming of Oncogene Expression in Gingival Mesenchymal Stem Cells Following Long-Term Culture In Vitro
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DOI：
10.1089/cell.2016.0056
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发表时间：
2017-06-01
影响因子：
1.6
通讯作者：
Trubiani, Oriana
中科院分区：
文献类型：
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作者：
Gugliandolo, Agnese;Rajan, Thangavelu Soundara;Trubiani, Oriana
间充质干细胞（MSC）是一种有前途的干细胞治疗资源，用于治疗不同的神经退行性疾病。特别是，牙齿间充质干细胞，鉴于其起源于神经嵴和其倾向于神经元分化，可能更适合移植。然而，MSCs是否能自发转化并诱发肿瘤仍存在争议。关于体外扩增后MSC中癌基因转录调控的数据不可用。在这项工作中，我们比较了牙龈来源的MSC在第10和41代的癌基因的基因表达水平。我们发现，22个癌基因的表达被废除牙龈骨髓间充质干细胞在第41代相比，在第10代，这可能表明一个更大的安全性，高数量的传代骨髓间充质干细胞。
Mesenchymal stem cells (MSCs) are a promising resource for stem cell therapy for the treatment of different neurodegenerative disorders. In particular, dental MSCs, given their origin from neural crest and their proneness toward neuronal differentiation, may be more suitable for transplantation. However, if MSCs can undergo spontaneous transformation and give rise to tumor is still debated. Data about transcriptional regulation of oncogenes in MSCs following in vitro expansion are not available. In this work, we compared gene expression levels of oncogenes in gingival-derived MSCs at passage number 10 and 41. We found that the expression of 22 oncogenes was abolished in gingival MSCs at passage number 41 compared to those at passage number 10, and this may indicate a greater safety of high number passage MSCs.