Characterization of serum miRNAs as molecular biomarkers for acute Stanford type A aortic dissection diagnosis.
Characterization of serum miRNAs as molecular biomarkers for acute Stanford type A aortic dissection diagnosis.
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血清 miRNA 作为急性斯坦福 A 型主动脉夹层诊断分子生物标志物的表征
DOI：
10.1038/s41598-017-13696-3
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发表时间：
2017-10-20
影响因子：
4.6
通讯作者：
Jiang X
中科院分区：
文献类型：
--
作者：
Xu Z;Wang Q;Pan J;Sheng X;Hou D;Chong H;Wei Z;Zheng S;Xue Y;Zhou Q;Cao H;Zhang CY;Wang D;Jiang X
由于急性斯坦福 A 型主动脉夹层 (AAAD) 患者在 48 小时内死亡率很高，因此迫切需要早期、便捷的诊断。循环 microRNA (miRNA) 是有前景的心血管疾病生物标志物，然而，人们对参与 AAAD 的循环 miRNA 知之甚少。此处，血清样本取自 104 名 AAAD+ 患者和 103 名年龄匹配的捐献者。使用 TaqMan 低密度阵列进行初步筛选，然后进行 RT-qPCR 确认。根据两阶段选择和验证过程，我们发现与 AAAD- 个体相比，AAAD+ 血清样本中 miR-25、miR-29a 和 miR-155 显着升高，而 miR-26b 显着降低。最重要的是，对于患有高血压的个体（这是 AAAD 的一个主要因素），4-miRNA 组合在预测那些更有可能患 AAAD 的人群方面也表现出很高的准确性。在盲试验组中，专家组正确分类了高血压队列中 93.33% 的 AAAD+ 患者和 86.67% 的对照组。最后，回顾性分析支持了用于早期 AAAD 检测的基于血清 miRNA 的生物标志物。总而言之，我们确定了 4-miRNA 的独特特征，可以作为 AAAD 诊断的无创生物标志物，特别是对于高血压患者。
Early and convenient diagnosis is urgently needed for acute Stanford type A aortic dissection (AAAD) patients due to its high mortality within the first 48 hours. Circulating microRNAs (miRNAs) are promising biomarkers of cardiovascular diseases, however, little is known about circulating miRNAs involved in AAAD. Here, the blood serum was sampled from 104 AAAD+ patients and 103 age-matched donors. Initial screening was conducted using the TaqMan Low Density Array followed by RT-qPCR confirmation. According to the two-phase selection and validation process, we found that miR-25, miR-29a and miR-155 were significantly elevated, while miR-26b was markedly decreased in AAAD+ serum samples compared with AAAD− individuals. Most importantly, for individuals with hypertension, which is a major contributor to AAAD, the 4-miRNA panel also showed high accuracy in predicting those who are more likely to develop AAAD. In the blind trial set, the panel correctly classified 93.33% AAAD+ patients and 86.67% controls from the hypertension cohort. Finally, the serum miRNA-based biomarker for early AAAD detection was supported by a retrospective analysis. Taken together, we identify a distinct profile of 4-miRNA that can serve as a noninvasive biomarker for AAAD diagnosis, especially for those with hypertension.
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影响因子：
6
作者：
Ikonomidis, John S.;Ivey, Charlotte R.;Wheeler, Jason B.;Akerman, Adam W.;Rice, Allison;Patel, Risha K.;Stroud, Robert E.;Shah, Asad A.;Hughes, Chad G.;Ferrari, Giovanni;Mukherjee, Rupak;Jones, Jeffrey A.
通讯作者：
Jones, Jeffrey A.
影响因子：
5.6
作者：
Leeper, Nicholas J.;Raiesdana, Azad;Kojima, Yoko;Chun, Hyung J.;Azuma, Junya;Maegdefessel, Lars;Kundu, Ramendra K.;Quertermous, Thomas;Tsao, Philip S.;Spin, Joshua M.
通讯作者：
Spin, Joshua M.
影响因子：
120.7
作者：
Hagan, PG;Nienaber, CA;Eagle, KA
通讯作者：
Eagle, KA
胸主动脉夹层与正常胸主动脉之间的 microRNA 谱比较表明 microRNA 在胸主动脉夹层发病机制中的潜在作用
影响因子：
4.3
作者：
Liao, Mingfang;Zou, Sili;Jing, Zaiping
通讯作者：
Jing, Zaiping
影响因子：
8.3
作者：
Bertoia, Monica L.;Waring, Molly E.;Eaton, Charles B.
通讯作者：
Eaton, Charles B.