MUSCLE REGENERATION FOLLOWING INJURY CAN BE MODIFIED IN-VIVO BY IMMUNE NEUTRALIZATION OF BASIC FIBROBLAST GROWTH-FACTOR, TRANSFORMING GROWTH-FACTOR-BETA-1 OR INSULIN-LIKE GROWTH-FACTOR-I
MUSCLE REGENERATION FOLLOWING INJURY CAN BE MODIFIED IN-VIVO BY IMMUNE NEUTRALIZATION OF BASIC FIBROBLAST GROWTH-FACTOR, TRANSFORMING GROWTH-FACTOR-BETA-1 OR INSULIN-LIKE GROWTH-FACTOR-I
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DOI：
10.1016/0165-5728(94)00166-l
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发表时间：
1995-03-01
影响因子：
3.3
通讯作者：
SEBILLE, A
中科院分区：
文献类型：
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作者：
LEFAUCHEUR, JP;SEBILLE, A
既往体外研究指出碱性成纤维细胞生长因子（bFGF）、转化生长因子β -1 （TGF β 1）、胰岛素样生长因子i （IGF-I）等多种生长因子对肌原性前体细胞增殖、分化和融合的作用。我们试图通过作用于可能参与这一过程的生长因子来改变小鼠指长伸肌去神经断流后的肌肉再生。在病变时向肌肉中注射抗bFGF或IGF-I的中和抗体，可减少再生肌纤维的数量和直径，表明活化卫星细胞的增殖和/或融合延迟。TGF β 1的中和作用导致小再生肌纤维数量增加，这可能是由于剩余的生长因子（即bFGF和IGF-I）对成肌细胞增殖的促进作用。这些对比结果强烈表明，生长因子调节体内肌肉再生，并将成为未来治疗肌肉疾病的可行工具。
Previous in vitro studies pointed out the role played by several growth factors (basic fibroblast growth factor or bFGF, transforming growth factor beta-1 or TGF beta 1, insulin-like growth factor-I or IGF-I) on the proliferation, the differentiation and the fusion of myogenic precursor cells. We attempted to modify the muscle regeneration which follows the denervation-devascularization of extensor digitorum longus in mice, by acting on the growth factors which are possibly involved in this process. The injection of neutralizing antibodies against either bFGF or IGF-I into the muscle at the time of lesion reduced the number and diameter of regenerating myofibres, suggesting a delay in proliferation and/or fusion of activated satellite cells. The neutralization of TGF beta 1 led to an increased number of small regenerating myofibres, which would be due to the promoting effects of the remaining growth factors (i.e. bFGF and IGF-I) on myoblast proliferation. These contrasted results strongly suggest that the growth factors regulate in vivo muscle regeneration and would be accessible tools for future therapy of muscular disorders.