ZONE ELECTROPHORESIS IN STARCH GELS - GROUP VARIATIONS IN THE SERUM PROTEINS OF NORMAL HUMAN ADULTS
ZONE ELECTROPHORESIS IN STARCH GELS - GROUP VARIATIONS IN THE SERUM PROTEINS OF NORMAL HUMAN ADULTS
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DOI：
10.1042/bj0610629
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发表时间：
1955-01-01
影响因子：
4.1
通讯作者：
SMITHIES, O
中科院分区：
文献类型：
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作者：
SMITHIES, O
本文介绍了一种以淀粉凝胶为支持介质的区带电泳方法。蛋白质检测通过染色进行。在许多情况下，其分辨能力上级于Tiselius方法。如果需要，可以使用少至0.02 ml的样品，并且该方法非常适合于比较密切相关的样品。当该方法用于正常人血清的电泳时，可以证明几种以前未描述的不可透析组分。这些成分可以在几种缓冲液中看到，包括巴比妥酸盐。通常使用硼酸盐缓冲液。电泳时间为16小时。排除电泳异常、凝血过程产物和意外溶血作为新组分来源。根据某些新成分的出现，发现40多名正常成人男女的血清可分为3组。各组的性别分布没有差异。表征这3组的组分具有在电泳条件下结合血红蛋白的共同性质，而不是任何其他血清蛋白共有的性质。当使用滤纸电泳时，它们迁移在α 2-球蛋白位置。特征蛋白的出现可能与年龄和遗传因素有关。新方法的高分辨率和它的局限性的可能原因进行了讨论。
A method of zone electrophoresis in which starch gel is used as the supporting medium is described. Protein detection is by staining. The resolving power is in many cases superior to that obtained by the Tiselius method. As little as 0.02 ml of sample can be used, if necessary, and the method is very well adapted to comparing closely related samples. When the method is used for the electrophoresis of normal human sera several previously undescribed non-dialyzable components may be demonstrated. These components can be seen with several buffers, including barbiturate. A borate buffer is normally employed. The period of electrophoresis is 16 hours. Electrophoretic anomalies, products of the clotting process, and inadvertent hemolysis as sources of the new components are excluded. Sera from over 40 normal human adults of both sexes are found to fall into 3 groups on the basis of the occurrence of some of the new components. No difference in the distribution of the groups in the sexes was shown. The components characterizing the 3 groups have a common property, not shared by any other of the serum proteins, of binding hemoglobin under the conditions used for electrophoresis. They migrate in the a2-globulin position when filter-paper electrophoresis is used. The occurrence of the characteristic proteins may be related to age and to hereditary factors. Possible reasons for the high resolving power of the new method and its limitations are discussed.