O-3-INDUCED CHANGE IN BRONCHIAL REACTIVITY TO METHACHOLINE AND AIRWAY INFLAMMATION IN HUMANS
O-3-INDUCED CHANGE IN BRONCHIAL REACTIVITY TO METHACHOLINE AND AIRWAY INFLAMMATION IN HUMANS
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DOI：
10.1152/jappl.1986.60.4.1321
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发表时间：
1986-04-01
影响因子：
3.3
通讯作者：
BOUSHEY, HA
中科院分区：
文献类型：
--
作者：
SELTZER, J;BIGBY, BG;BOUSHEY, HA
犬暴露于O3诱导的气道反应性增加与气道上皮炎症相关，这可通过上皮活检和支气管肺泡灌洗液中发现的中性粒细胞（多形核白细胞）数量增加来证明。我们调查了10名健康的人类受试者是否O3诱导的高反应性同样与气道炎症通过检查暴露于空气或O3（0.4或0.6 ppm）后获得的支气管肺泡灌洗液中回收的细胞类型的变化。我们还测定了灌洗液中花生四烯酸的环氧合酶和脂氧合酶代谢产物的浓度。我们在每次暴露前和暴露后测量气道对吸入乙酰甲胆碱气雾剂的反应性，并在3小时后进行支气管肺泡灌洗。我们发现更多的中性粒细胞在灌洗液中的O3暴露的主题，特别是在那些在O3暴露产生气道反应性增加。我们还发现，E2，F2 α，和血栓素B2。这些结果表明，在人类受试者中，O3-诱导的乙酰甲胆碱高反应性与中性粒细胞流入气道和花生四烯酸的一些环氧合酶代谢物水平的变化有关。
The increase in airway responsiveness induced by O3 exposure in dogs is associated with airway epithelial inflammation, as evidenced by an increase in the number of neutrophils (polymorphonuclear leukocytes) found in epithelial biopsies and in bronchoalveolar lavage fluid. We investigated in 10 healthy, human subjects whether O3-induced hyperresponsiveness was similarly associated with airway inflammation by examining changes in the types of cells recovered in bronchoalveolar lavage fluid obtained after exposure to air or to O3 (0.4 or 0.6 ppm). We also measured the concentrations of cyclooxygenase and lipoxygenase metabolites of arachidonic acid in lavage fluid. We measured airway responsiveness to inhaled methacholine aerosol before and after each exposure and performed bronchoalveolar lavage 3 h later. We found more neutrophils in the lavage fluid from O3-exposed subjects, especially in those in whom O3 exposure produced an increase in airway responsiveness. We also found significant increases in the concentrations of prostaglandins E2, F2.alpha., and thromboxane B2 in lavage fluid from O3-exposed subjects. These results show that in human subjects O3-induced hyperresponsiveness to methacholine is associated with an influx of neutrophils into the airways and with changes in the levels of some cyclooxygenase metbolites of arachidonic acid.