Increased release of ATP from endothelial cells during acute inflammation
Increased release of ATP from endothelial cells during acute inflammation
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DOI：
10.1007/s000110050341
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发表时间：
1998-08-01
影响因子：
6.7
通讯作者：
Burnstock, G
中科院分区：
文献类型：
--
作者：
Bodin, P;Burnstock, G
目标与设计：方法：采用锥板剪切应力仪对原代培养的人脐静脉内皮细胞（HUVEC）施加剪切应力，观察剪切应力对细胞分泌三磷酸腺苷（ATP）的影响。结果：在单独的剪切应力（25达因/cm（2））条件下，ATP在培养基中积累，并在刺激3 ~ 5分钟后达到最大值（121.7 +/- 13.2 pmol/ml）。内皮细胞与50 μ g/ml（314.4 +/- 26.7 pmol/ml）和10 μ g/ml脂多糖（207.7 +/- 22.2 pmol/ml）预孵育4小时后，剪切应力刺激的ATP释放显著增加。地塞米松，抗炎糖皮质激素，抑制脂多糖的影响。结论：这些结果表明，未受损的内皮细胞释放ATP在实验性炎症条件下，并支持细胞外ATP在炎症过程中的早期作用。
Objective and Design: The effects of lipopolysaccharide (LPS), a potent inflammatory mediator, on the shear stress stimulated release of adenosine triphosphate (ATP) were investigated on endothelial cells from human umbilical Vein in primary culture.Methods: Human umbilical vein endothelial cells (HUVEC) in primary cultures were subjected to shear stress using a cone and plate apparatus. ATP released by the cells was measured by luminometry, using a luciferin-luciferase assay.Results: Under conditions of shear stress alone (25 dyn/cm(2)), ATP accumulates into the culture medium and reaches a maximum after 3 to 5 min of stimulation (121.7 +/- 13.2 pmol/ml). The shear stress-stimulated release of ATP was significantly increased after a 4 h pre-incubation of endothelial cells with 50 mu g/ml (314.4 +/- 26.7 pmol/ml) and 10 mu g/ml lipopolysaccharide (207.7 +/- 22.2 pmol/ml). Dexamethasone, an anti-inflammatory glucocorticoid, inhibited the effects of lipopolysaccharide.Conclusions: These results show that non-damaged endothelial cells release ATP under experimental inflammatory conditions and support an early role of extracellular ATP in the inflammatory process.