Genetic characterization of the rotavirus nonstructural protein, NSP4
Genetic characterization of the rotavirus nonstructural protein, NSP4
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DOI：
10.1006/viro.1997.8727
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发表时间：
1997-09-29
期刊：
影响因子：
3.7
通讯作者：
Palombo, EA
中科院分区：
文献类型：
--
作者：
Kirkwood, CD;Palombo, EA
轮状病毒非结构蛋白NSP4在病毒组装中发挥作用，作为单壳颗粒的细胞内受体，并协助这些颗粒在内质网中的易位。最近，NSP4被认为与轮状病毒毒力有关，并被认为是一种肠毒素，通过钙依赖性信号转导途径触发氯化物分泌。有限序列分析显示NSP4是一个保守性较好的蛋白。为了确定NSP4基因编码的序列变异程度，我们从9个人类轮状病毒株中对该基因进行了测序。这些数据以及对其他人轮状病毒株和多种动物轮状病毒（牛、猴、马和猪）的Northern blot杂交分析表明，轮状病毒株中存在三种NSP4基因型，并且NSP4基因型与VP6亚群之间存在相关性。在标准人轮状病毒和当地社区流行的毒株中发现了两个不同的NSP4基因（编码不同类型的NSP4蛋白），这些基因与一些动物毒株中的同源基因具有同源性。(C) 1997学术出版社。
The rotavirus nonstructural protein NSP4 plays a role in viral assembly by acting as an intracellular receptor for single-shelled particles and assisting in the translocation of these particles across the endoplasmic reticulum. Recently, NSP4 has been implicated in rotavirus virulence and is thought to act as an enterotoxin which triggers chloride secretion by a calcium-dependent signal transduction pathway. Limited sequence analysis of NSP4 shows a well-conserved protein. To define the extent of sequence variation in the gene coding for NSP4, we have sequenced this gene from nine human rotavirus strains. These data and the analysis of additional human strains and various animal rotaviruses (bovine, simian, equine, and porcine) by Northern blot hybridization suggested that three NSP4 genotypes were present among rotavirus strains, A correlation between NSP4 genotype and VP6 subgroup was also implied. Two different NSP4 genes (which encoded distinct types of NSP4 proteins) were found among standard human rotaviruses and in strains circulating in the local community and these showed homology to cognate genes in some animal strains. (C) 1997 Academic Press.