Effects of recombinant goose adiponectin on steroid hormone secretion in Huoyan geese ovarian granulosa cells
Effects of recombinant goose adiponectin on steroid hormone secretion in Huoyan geese ovarian granulosa cells
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DOI：
10.1016/j.anireprosci.2019.03.019
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发表时间：
2019-06-01
影响因子：
2.2
通讯作者：
Luan, Xinhong
中科院分区：
文献类型：
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作者：
Meng, Bo;Cao, Zhongzan;Luan, Xinhong
脂联素是一种与生殖过程调节相关的脂肪因子。为了确定重组鹅脂联素是否有助于鹅卵巢甾体生成，采用重组DNA技术制备了重组鹅脂联素蛋白。研究了重组脂联素对霍岩鹅卵巢颗粒细胞孕酮（P-4）和雌二醇（E-2）产生的影响。此外，研究了重组脂联素（2.5 μ g/mL）对颗粒细胞中甾体急性调节蛋白StAR、细胞色素P450scc、胆固醇侧链切割酶CYP11A1和细胞色素P450芳香化酶CYP19A1 mRNA和蛋白丰度的影响。结果表明，重组脂联素（2.5 μ g/mL）对鹅卵巢颗粒细胞P-4和E-2的产生有明显的刺激作用（P < 0.01），对E-2的产生有微弱的抑制作用（P < 0.05）。与未加脂联素组比较，经脂联素处理后，StAR和CYP11A1 mRNA的丰度升高（P < 0.05）， CYP19A1 mRNA的丰度略有降低（P < 0.05）。此外，SCAR的荧光强度较pbs处理组（P < 0.05）和对照组（P < 0.05）有增大趋势，StAR蛋白丰度较其他两组增大（P < 0.05）。脂联素处理后，CYP11A1的荧光强度和蛋白丰度增加（P < 0.05），而CYP19A1的荧光强度和蛋白丰度有降低的趋势（P < 0.05）。结果表明，重组鹅脂联素影响鹅卵巢颗粒细胞的甾体生成和/或激素分泌。因此，脂联素在鹅的生殖生理中可能具有重要的功能。
Adiponectin is an adipokine associated with the regulation of reproductive processes. To determine whether recombinant goose adiponectin contributes to the goose ovarian steroidogenesis, the native and then purified goose recombinant adiponectin protein was produced using recombinant DNA technologies. The effect of recombinant adiponectin on the progesterone (P-4) and estradiol (E-2) production in Huoyan geese ovarian granulosa cells was examined. Furthermore, the effect of recombinant adiponectin (2.5 mu g/mL) on the abundance of StAR (steroidogenic acute regulatory protein), CYP11A1 (cytochrome P450scc, cholesterol side-chain cleavage enzyme) and CYP19A1 (cytochrome P450 aromatase) mRNA and protein in granulosa cells was evaluated. Results indicate that a 24-h treatment with recombinant adiponectin (2.5 mu g/mL) affected P-4 and E-2 production by geese ovarian granulosa cells by stimulating P-4 production (P < 0.01) and weakly inhibiting E-2 production (P > 0.05). Furthermore, when the results with treatment were compared to when there was not adiponectin treatment, the abundance of StAR and CYP11A1 mRNA was greater (P < 0.05) while the CYP19A1 mRNA slightly decreased (P > 0.05). In addition, the fluorescence intensity of SCAR tended to be greater compared to PBS-treated (P < 0.05) and control groups (P > 0.05) and StAR protein abundance was greater (P < 0.05) compared to the other two groups. The fluorescence intensity and protein abundances of CYP11A1 increased (P < 0.05) while those for CYP19A1 tended to decrease (P > 0.05) after adiponectin treatment. The results indicate recombinant goose adiponectin affects steroidogenesis and/or hormone secretion of geese ovarian granulosa cells. There may, therefore, be important functions of adiponectin in goose reproductive physiology.