Distinctiveness and continuity in transcriptome and connectivity in the anterior-posterior axis of the paraventricular nucleus of thalamus
Distinctiveness and continuity in transcriptome and connectivity in the anterior-posterior axis of the paraventricular nucleus of thalamus
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DOI：
10.1101/2022.02.13.480207
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发表时间：
2022-02
期刊：
影响因子：
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通讯作者：
Yasuyuki Shima;Henrik Skibbe;Yohei Sasagawa;Noriko Fujimori;I. Nikaido;N. Hattori;Tadafumi Kato
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文献类型：
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作者：
Yasuyuki Shima;Henrik Skibbe;Yohei Sasagawa;Noriko Fujimori;I. Nikaido;N. Hattori;Tadafumi Kato
丘脑室旁核（PVT）将轴突投射到多个区域并介导广泛的行为。在PVT中的功能和轴突投射的异质性已被报道，但在PVT中存在什么样的细胞类型以及它们之间的差异还没有得到解决。我们应用单细胞RNA测序来描述小鼠PVT神经元的转录组学特征。PVT神经元的转录组具有连续分布，其中前后轴对应的方差最大。尽管单细胞转录组将PVT神经元分为四种类型，但转录组和组织学分析显示其连续性。同样，前和后亚群几乎不重叠的轴突投射模式，而另一个人口显示中间模式。此外，他们对食欲相关神经肽的反应不同，它们的化学发生激活在食物消耗中表现出相反的作用。我们的研究显示了PVT神经元作为行为调节中枢的功能的对比和连续性。
The paraventricular nucleus of the thalamus (PVT) projects axons to multiple areas and mediates a wide range of behaviors. Heterogeneity of functions and axonal projections in PVT have been reported, but what cell types exist in PVT and how different they are have not been addressed. We applied single-cell RNA sequencing to depict transcriptomic characteristics of mouse PVT neurons. The transcriptome of PVT neurons had a continuous distribution with the largest variance corresponding to the anterior-posterior axis. Although the single-cell transcriptome classified PVT neurons into four types, transcriptomic and histological analyses showed their continuity. Similarly, anterior and posterior subpopulations had nearly non-overlapping axon projection patterns, while another population showed intermediate patterns. In addition, they responded differently to appetite-related neuropeptides, and their chemogenetic activation showed opposing effects in food consumption. Our studies showed contrasts and continuity of PVT neurons underlying their function as a behavior-modulating hub.