LENS MEMBRANES .7. MIP IS AN IMMUNOLOGICALLY SPECIFIC COMPONENT OF LENS FIBER MEMBRANES AND IS IDENTICAL WITH 26K-BAND PROTEIN
LENS MEMBRANES .7. MIP IS AN IMMUNOLOGICALLY SPECIFIC COMPONENT OF LENS FIBER MEMBRANES AND IS IDENTICAL WITH 26K-BAND PROTEIN
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DOI：
10.1016/0014-4835(79)90009-5
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发表时间：
1979-01-01
影响因子：
3.4
通讯作者：
WINKENS, HJ
中科院分区：
文献类型：
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作者：
BROEKHUYSE, RM;KUHLMANN, ED;WINKENS, HJ
小牛晶状体MIP[主要内源多肽](从纤维膜中提取)和26,000条带蛋白(经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法分离)具有非常相似的氨基酸组成和相对分子质量。MIP由两个免疫同源组分组成，与26,000条带蛋白中检测到的两个抗原相同。MIP提取物含有两种极少量的抗原，这在尿素处理的纤维和上皮晶状体膜中是常见的。26,000条带的蛋白包含其中一种抗原。免疫扩散、免疫荧光和SDS-PAGE表明，MIP是纤维膜中的一种特殊成分，在上皮膜中未检测到。MIP是分化的标志。MIP决定簇仅存在于晶状体中，显示了蛋白质的器官特异性。
Calf lens MIP [main intrinsic polypeptide] (solvent-extracted from the fiber membranes) and 26,000 band protein (isolated by SDS-PAGE [sodium dodecyl sulfate-polyacrylamide gel electrophoresis]) had very similar amino acid compositions and MW. MIP comprises 2 components immunlogically identrical with the 2 antigens detected in 26,000 band protein. MIP extracts contain 2 antigens in very small amounts which are common to urea-treated fiber and epithelial lens membranes. The 26,000 band protein contains 1 of these antigens. Immunodiffusion, immunofluorescence and SDS-PAGE show MIP is a specific component of the fiber membranes, which is not detected in epithelial membranes. MIP is a marker of differentiation. MIP determinants only occur in the lens demonstrating the organ specificity of the protein.