DIFFERENTIAL EXPRESSION OF TYPE-2 AND TYPE-3 INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR MESSENGER-RNAS IN VARIOUS MOUSE-TISSUES - IN-SITU HYBRIDIZATION STUDY
DIFFERENTIAL EXPRESSION OF TYPE-2 AND TYPE-3 INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR MESSENGER-RNAS IN VARIOUS MOUSE-TISSUES - IN-SITU HYBRIDIZATION STUDY
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DOI：
10.1007/s004410050345
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发表时间：
1995-05-01
影响因子：
3.6
通讯作者：
MIKOSHIBA, K
中科院分区：
文献类型：
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作者：
FUJINO, I;YAMADA, N;MIKOSHIBA, K
肌醇1，4，5-三磷酸受体（IP（3）R）是细胞内负责动员储存的Ca 2+的Ca 2+释放通道。三种不同的受体类型已被分子克隆，它们的基因已被归类为一个家族。1型受体（IP（3）R1）的基因主要在小脑浦肯野神经元中表达，但其基因产物广泛存在于各种组织中;然而，关于哪种类型的细胞表达另外两种受体类型，2型和3型（分别为IP（3）R2和IP（3）R3）的信息很少。我们用原位杂交组织化学方法研究了IP（3）R基因家族在小鼠不同组织中的表达。与IP（3）R1相比，IP（3）R2和IP（3）R3 mRNA在所有检测组织中的表达水平均较低。IP（3）R2 mRNA定位于下颌下腺小叶内导管细胞、肾脏肾小管细胞、附睾管上皮细胞和卵巢滤泡颗粒细胞，IP（3）R3 mRNA定位于胃细胞、唾液腺和胰腺腺泡细胞以及小肠上皮细胞。已知表达IP（3）R2或IP（3）R3 mRNA的所有这些细胞具有分泌功能，其中已显示涉及IP 3/Ca 2+信号传导，因此IP（3）R2或IP（3）R3可能是这些细胞中分泌的先决条件。
The inositol 1,4,5-trisphosphate receptor (IP(3)R) is an intracellular Ca2+ release channel responsible for mobilizing stored Ca2+. Three different receptor types have been molecularly cloned, and their genes have been classified into a family. The gene for the type 1 receptor (IP(3)R1) is predominantly expressed in cerebellar Purkinje neurons, but its gene product is localized widely in a variety of tissues; however, there is little information on what types of cells express the other two receptor types, type 2 and type 3 (IP(3)R2 and IP(3)R3, respectively). We studied the expression of the IP(3)R gene family in various mouse tissues by in situ hybridization histochemistry. Compared with IP(3)R1, the levels of expression of IP(3)R2 and IP(3)R3 mRNAs were low in all of the tissues tested. IP(3)R2 mRNA was localized in the intralobular duct cells of the submandibular gland, the urinary tubule cells of the kidney, the epithelial cells of epididymal ducts and the follicular granulosa cells of the ovary, while the IP(3)R3 mRNA was distributed in gastric cells, salivary and pancreatic acinar cells and the epithelium of the small intestine. All of these cells which express either IP(3)R2 or IP(3)R3 mRNA are known to have a secretory function in which IP3/Ca2+ signalling has been shown to be involved, and thus either IP(3)R2 or IP(3)R3 may be a prerequisite to secretion in these cells.