Radix Paeoniae Rubra and Radix Paeoniae Alba Attenuate CCl4-induced acute liver injury: an ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) based metabolomic approach for the pharmacodynamic study of Traditional Chinese Medicines (TCMs).
Radix Paeoniae Rubra and Radix Paeoniae Alba Attenuate CCl4-induced acute liver injury: an ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) based metabolomic approach for the pharmacodynamic study of Traditional Chinese Medicines (TCMs).
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DOI：
10.3390/ijms131114634
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发表时间：
2012-11-09
影响因子：
5.6
通讯作者：
Yang L
中科院分区：
文献类型：
--
作者：
Wang R;Xiong AZ;Teng ZQ;Yang QW;Shi YH;Yang L
代谢组学在药效学研究中得到了广泛的应用，尤其是在中药方面。白芍和赤芍在中医中应用广泛，两者都具有保肝作用。本研究建立了CCl4诱导的大鼠急性肝损伤模型，并通过观察血清转氨酶活性进行了验证。应用代谢组学方法研究白芍、赤芍对急性肝损伤大鼠代谢变化的影响。大鼠血清的偏最小二乘判别分析(偏最小二乘判别分析)及其超高效液相色谱-质谱仪(UPLC-MS)指纹图谱可以区分给药后的对照组和急性肝损伤模型大鼠。时间依赖的偏最小二乘-多巴胺曲线显示，给予中药的大鼠在接受CCl4注射后的2小时内，代谢模式的变化已趋于稳定。提示中药对小鼠肝损伤有保护作用。检测和鉴定了几个潜在的生物标志物，包括肌酸、脱氧胆酸、胆碱、5-亚甲基四氢叶酸、叶酸和甘胆酸。并对这些代谢变化的生理意义进行了讨论。
Metabolomics has been frequently used in pharmacodynamic studies, especially those on traditional Chinese medicine (TCM). Radix Paeoniae Alba and Radix Paeoniae Rubra are popularly used in TCM, and both have hepatoprotective effects. In this study, a CCl4-induced acute liver injury rat model was established and confirmed by the observed serum aminotransferase activities. The metabolomics approach was applied to study the influence of Radix Paeoniae Alba and Radix Paeoniae Rubra on the metabolic changes in rats with acute liver injury. The partial least-squares-discriminant analysis (PLS-DA) of rat serum and their ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) fingerprints allowed discrimination of controlled, acute liver injury-model rats after administration of the two types of TCMs. The time-dependent PLS-DA plots showed that the changes in the metabolic patterns of the rats, which were administered with the TCMs, had stabilized within 2 h after they received the intraperitoneal CCl4 injection. The results indicated the protective effect of TCMs against liver injury. Several potential biomarkers were detected and identified, which included creatine, deoxycholic acid, choline, 5-methylenetetrahydrofolate, folic acid, and glycocholic acid. The physiological significance of these metabolic changes was discussed.
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