Selective BuChE inhibitory activity, chemical composition, and enantiomer content of the volatile oil from the Ecuadorian plant Clinopodium brownei
Selective BuChE inhibitory activity, chemical composition, and enantiomer content of the volatile oil from the Ecuadorian plant Clinopodium brownei
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DOI：
10.1016/j.bjp.2019.08.001
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发表时间：
2019-12-01
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影响因子：
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通讯作者：
Armijos, Chabaco
中科院分区：
文献类型：
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作者：
Matailo, Andrea;Bec, Nicole;Armijos, Chabaco
本文报道了风轮菜挥发油的化学组成和对映体含量。Kuntze，唇形科。该植物在厄瓜多尔南部采集。经GC-MS联用鉴定出31种成分，占挥发油总量的96.15%。主要成分为胡薄荷酮（48.44%）、薄荷酮（34.55%）和β-菖蒲烯醇（3.41%）。含氧单萜（86.06%），其次是含氧倍半萜（5.36%）构成了最丰富的馏分。对映体选择性GC-MS法测定了β-蒎烯、桧烯、3-辛醇、薄荷酮、胡薄荷酮和乙酸薄荷酯的对映体组成，其中（-）-藜芦酮的对映体过量最高（ee = 83.4%）。在体外试验中，挥发油显示出对丁酰胆碱酯酶的高选择性抑制活性，IC 50为13.4 +/- 1.8 μ g/ml。相反，它对乙酰胆碱酯酶的活性较弱，IC 50>250 μ g/ml。(C)2019年，巴西制药公司。出版社：Elsevier Editora Ltda
This paper describes the chemical composition and the enantiomer content of the volatile oil hydrodistilled from Clinopodium brownei (Sw.) Kuntze, Lamiaceae. The plant was collected in the South of Ecuador. Thirty one components were identified by GC-MS, which accounted for the 96.15% of the volatile oil. The major components were pulegone (48.44%), menthone (34.55%) and beta-acorenol (3.41%). Oxygenated monoterpenes (86.06%), followed by oxygenated sesquiterpenes (5.36%) constituted the most abundant fractions. The enantiomeric compositions of beta-pinene, sabinene, 3-octanol, menthone, pulegone and menthyl acetate were determined by enantioselective GC-MS. (-)-Menthone showed the highest enantiomeric excess (ee = 83.4%). In in vitro tests, the volatile oil showed high selective inhibitory activity for butyrylcholinesterase with an IC50, 13.4 +/- 1.8 mu g/ml. In contrast, it was weakly active against acetylcholinesterase with an IC50 >250 mu g/ml. (C) 2019 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda.