On-site cell concentration and viability detections using smartphone based field-portable cell counter
On-site cell concentration and viability detections using smartphone based field-portable cell counter
复制标题
使用基于智能手机的现场便携式细胞计数器进行现场细胞浓度和活力检测
DOI：
10.1016/j.aca.2019.05.029
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发表时间：
2019
影响因子：
6.2
通讯作者：
Fei Liu
中科院分区：
文献类型：
--
作者：
Wenjie Kang;Huachuan Huang;Ming Cai;Yangyang Li;Wei Hou;Feng Yun;Xuping Wu;Liang Xue;Shouyu Wang;Fei Liu
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We designed a smartphone based field-portable cell counter combining the smartphone microscope for bright-field image recording and the smartphone application for automatically cell recognition, counting and analysis. To our best knowledge, it is the first time that a smartphone based cell counter can distinguish and count both live and dead cells simultaneously. Compared to the results obtained by hemocytometer, commercial cell counter and flow cytometer, the proposed device was proved to detect cell concentration and viability accurately within the application range between 105cells/mL and 107cells/mL. Though multiple fields of view were measured to increase the sampling amount for error reduction, the whole operations including image recording and processing can still be finished rapidly. Moreover, the proposed device is cost-effective with small size of 170 mm × 113 mm × 168 mm containing a built-in power supply. Considering its advantages as high accuracy, fast speed, low cost, long battery life and compact configuration, it is believed the proposed device is a potential tool applied in on-site cell analysis.