REGULATION OF CUCUMBER CLASS-III CHITINASE GENE-EXPRESSION
REGULATION OF CUCUMBER CLASS-III CHITINASE GENE-EXPRESSION
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DOI：
10.1094/mpmi-7-0048
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发表时间：
1994-01-01
影响因子：
3.5
通讯作者：
RYALS, J
中科院分区：
文献类型：
--
作者：
LAWTON, KA;BECK, J;RYALS, J
对黄瓜酸性III类几丁质酶的染色体区域进行了分离和鉴定。由于明显的基因三重复制，病原体诱导基因（CHI2）两侧有两个密切相关的基因，具有完整的开放阅读框（ORF）。这三种orf的高度保守性表明，每种编码蛋白在植物生长发育中都起着重要作用。使用基因特异性探针和RNA- pcr分析每个基因的RNA的发育和组织特异性表达。并对各基因在不同诱导处理下的表达进行了表征。只检测到与CHI2对应的转录本。几丁质酶mRNA丰度在施用环己亚胺后略有升高；但环己亚胺处理抑制了水杨酸对其的诱导作用。
The chromosomal region encoding the acidic class III chitinase from cucumber has been isolated and characterized. As a result of an apparent gene triplication, the pathogen-induced gene (CHI2) is flanked by two closely related genes with complete open reading frames (ORF). The high level of conservation within the three ORFs suggests an essential role for each encoded protein in plant growth and development. The developmental and tissue-specific expression of RNA from each gene was analyzed using both gene-specific probes and RNA-PCR. The expression of each gene in response to various inducing treatments was also characterized. Only transcripts corresponding to CHI2 were detected. Chitinase mRNA abundance increased slightly following cycloheximide application; however, its potent induction by salicylic acid was inhibited by cycloheximide treatment.