Detection and evolutionary analysis of soybean miRNAs responsive to soybean mosaic virus
Detection and evolutionary analysis of soybean miRNAs responsive to soybean mosaic virus
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DOI：
10.1007/s00425-012-1835-3
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发表时间：
2013-01
期刊：
影响因子：
4.3
通讯作者：
Xia Yin;Jiao Wang;Hao Cheng;Xiaolin Wang;Deyue Yu
中科院分区：
文献类型：
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作者：
Xia Yin;Jiao Wang;Hao Cheng;Xiaolin Wang;Deyue Yu
MicroRNA（miRNA）是一类非编码RNA，在多种生物体中具有重要的基因调控作用。然而，参与大豆对大豆花叶病毒 (SMV) 反应的 miRNA 尚不清楚。为了鉴定参与大豆对 SMV 反应的新型 miRNA 和生物胁迫调节小 RNA，从模拟接种和 SMV 感染的大豆叶片构建了两个小 RNA 文库并进行了测序。这导致发现了179个miRNA，代表52个家族，其中属于3个家族的5个miRNA是大豆中的新miRNA。很大一部分（71.5%）的 miRNA 来自片段复制，类似于驱动蛋白质编码基因进化的过程。此外，我们预测了这些已识别 miRNA 的 346 个潜在靶点，并通过修改的 5'-RACE 分析验证了 12 个靶点。最后，观察到参与植物抗性的三种 miRNA（miR160、miR393 和 miR1510）对 SMV 感染作出反应。 miRNA 和抗性相关基因之间的相互作用为病原体逃避宿主识别提供了一种新机制。
MicroRNAs (miRNA) are a class of non-coding RNAs that have important gene regulatory roles in various organisms. However, the miRNAs involved in soybean’s response to soybean mosaic virus (SMV) are unknown. To identify novel miRNAs and biotic-stress regulated small RNAs that are involved in soybean’s response to SMV, two small RNA libraries were constructed from mock-inoculated and SMV-infected soybean leaves and sequenced. This led to the discovery of 179 miRNAs, representing 52 families, among which five miRNAs belonging to three families were novel miRNAs in soybean. A large proportion (71.5 %) of miRNAs arose from segmental duplication, similar to the process that drives the evolution of protein-coding genes. In addition, we predicted 346 potential targets of these identified miRNAs, and verified 12 targets by modified 5′-RACE analysis. Finally, three miRNAs (miR160, miR393 and miR1510) that are involved in plant resistance were observed to respond to SMV infection. The interaction between miRNAs and resistance-related genes provides a novel mechanism for pathogens to evade host recognition.