Pool of Resistance Mechanisms to Glyphosate in Digitaria insularis
Pool of Resistance Mechanisms to Glyphosate in Digitaria insularis
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DOI：
10.1021/jf204089d
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发表时间：
2012-01-18
影响因子：
6.1
通讯作者：
Luque de Castro, Maria Dolores
中科院分区：
文献类型：
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作者：
de Carvalho, Leonardo Bianco;da Costa Aguiar Alves, Pedro Luis;Luque de Castro, Maria Dolores
在巴西已经检测到对草甘膦具有抗性的马唐生物型。在控制条件下进行研究，以确定吸收，转运，代谢和基因突变作为草甘膦抗性机制的作用。岛状的敏感的生物型吸收至少12%以上的C-14-草甘膦治疗后48小时（HAT）比抗性生物型。在12 HAT下观察到高差异C-14-草甘膦转运，使得>70%的吸收的除草剂保留在抗性生物型中的处理的叶片中，而在96 HAT下42%保留在敏感生物型中。在抗性生物型中，草甘膦降解为氨甲基膦酸（AMPA）、乙醛酸和肌氨酸的比例>90%，而敏感生物型降解了少量除草剂（高达11%），高达168 HAT。两个氨基酸的变化被发现在位置182和310在EPSPS，由脯氨酸苏氨酸和酪氨酸半胱氨酸取代，分别在抗性生物型。因此，吸收、转运、代谢和基因突变在D.抗草甘膦。
Digitaria insularis biotypes resistant to glyphosate have been detected in Brazil. Studies were carried out in controlled conditions to determine the role of absorption, translocation, metabolism, and gene mutation as mechanisms of glyphosate resistance in D. insularis. The susceptible biotype absorbed at least 12% more C-14-glyphosate up to 48 h after treatment (HAT) than resistant biotypes. High differential C-14-glyphosate translocation was observed at 12 HAT, so that >70% of the absorbed herbicide remained in the treated leaf in resistant biotypes, whereas 42% remained in the susceptible biotype at 96 HAT. Glyphosate was degraded to aminomethylphosphonic acid (AMPA), glyoxylate, and sarcosine by >90% in resistant biotypes, whereas a small amount of herbicide (up to 11%) was degraded by the susceptible biotype up to 168 HAT. Two amino acid changes were found at positions 182 and 310 in EPSPS, consisting of a proline to threonine and a tyrosine to cysteine substitution, respectively, in resistant biotypes. Therefore, absorption, translocation, metabolism, and gene mutation play an important role in the D. insularis glyphosate resistance.