Analysis of the activation status of Akt, NFκB, and Stat3 in human diffuse gliomas
Analysis of the activation status of Akt, NFκB, and Stat3 in human diffuse gliomas
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DOI：
10.1038/labinvest.3700123
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发表时间：
2004-08-01
影响因子：
5
通讯作者：
Fuller, GN
中科院分区：
文献类型：
--
作者：
Wang, HM;Wang, H;Fuller, GN
磷酸酶和张力蛋白同源物（PTEN）的缺失和表皮生长因子受体（EGFR）基因的扩增有助于胶质瘤的进展。Akt、NF κ B、信号转导子和转录激活子3（Stat3）作为PTEN和EGFR信号通路的下游靶点，在细胞增殖、凋亡和肿瘤发生中发挥重要作用。我们使用组织芯片和免疫组化检测了259例弥漫性胶质瘤中Akt、NF κ B和Stat3的激活状态，并评估了它们与胶质瘤分级的相关性。我们观察到Akt和NF κ B的活化状态与胶质瘤分级之间存在显著正相关。相比之下，仅观察到磷酸化Stat3的局灶性免疫反应性。
Loss of phosphatase and tensin homolog (PTEN) and amplification of the epidermal growth factor receptor (EGFR) gene contribute to the progression of gliomas. As downstream targets of the PTEN and EGFR signaling pathways, Akt, NFkappaB, and signal transducer and activator of transcription-3 (Stat3) have been shown to play important roles in the control of cell proliferation, apoptosis, and oncogenesis. We examined the activation status of Akt, NFkappaB, and Stat3 in 259 diffuse gliomas using tissue microarrays and immunohistochemistry, and evaluated their association with glioma grade. We observed significant positive correlations between the activation status of Akt and NFkappaB and glioma grade. In contrast, only focal immunoreactivity for phospho-Stat3 was observed in