IMMUNIZATION OF RABBITS WITH ENTEROTOXIGENIC ESCHERICHIA-COLI COLONIZATION FACTOR ANTIGEN (CFA/I) ENCAPSULATED IN BIODEGRADABLE MICROSPHERES OF POLY (LACTIDE-CO-GLYCOLIDE)
IMMUNIZATION OF RABBITS WITH ENTEROTOXIGENIC ESCHERICHIA-COLI COLONIZATION FACTOR ANTIGEN (CFA/I) ENCAPSULATED IN BIODEGRADABLE MICROSPHERES OF POLY (LACTIDE-CO-GLYCOLIDE)
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DOI：
10.1016/0264-410x(93)90012-m
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发表时间：
1993-01-01
期刊：
影响因子：
5.5
通讯作者：
LEWIS, DH
中科院分区：
文献类型：
--
作者：
EDELMAN, R;RUSSELL, RG;LEWIS, DH
我们已经寻找了一种有效的口服给药系统，用于纯化的产肠毒素大肠杆菌（ETEC）菌毛粘附素，CFA/I，其增强抗定植免疫。纯化的CFA/I抗原包封在可生物降解的聚合物微球的聚（DL-丙交酯-共-乙交酯）（PLG）诱导一个有力的，全身CFA/I IgG抗体反应，通过胃内管免疫兔一次;口服，未包封的CFA/I诱导很少或没有循环抗体。在喂食CFA/I-PLG微球疫苗的三只兔子中的一只中检测到CFA/I特异性S-IgA粪抗体。我们得出结论，PLG微球保护CFA/I免于在胃中降解，并有效地递送抗原以供宿主免疫系统处理。
We have searched for an effective oral delivery system for a purified enterotoxigenic Escherichia coli (ETEC) fimbrial adhesin, CFA/I, which elicits anti-colonization immunity. Purified CFA/I antigen encapsulated in biodegradable polymer microspheres of poly (DL-lactide-co-glycolide) (PLG) induced a vigorous, systemic CFA/I IgG antibody response in rabbits immunized once via intragastric tube; oral, unencapsulated CFA/I induced little or no circulating antibody. CFA/I-specific, S-IgA coproantibody was detected in one of three rabbits fed with the CFA/I-PLG microsphere vaccine. We conclude that PLG microspheres protected CFA/I from degradation in the stomach and effectively delivered the antigen for processing by the host's immune system.