Changes in equine intestinal stem/progenitor cell number at resection margins in cases of small intestinal strangulation.
Changes in equine intestinal stem/progenitor cell number at resection margins in cases of small intestinal strangulation.
复制标题
小肠绞窄病例中切除边缘的马肠干/祖细胞数量的变化。
DOI：
10.1111/evj.13927
复制
发表时间：
2023
影响因子：
2.2
通讯作者：
Gonzalez,LiaraM
中科院分区：
文献类型：
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作者：
Veerasammy,Brittany;Gonzalez,Gabriel;Báez-Ramos,Patricia;Schaaf,CeciliaR;Stewart,AmyStieler;Ludwig,ElsaK;McKinney-Aguirre,Caroline;Freund,John;Robertson,James;Gonzalez,LiaraM
背景肠上皮干细胞（ISC）是肠上皮再生的主要细胞，对肠损伤后恢复屏障功能至关重要。评估小肠绞窄（SIS）病例中ISC生物标志物表达可提供对临床进展的洞察。ObjectivesIntestinal切除边缘从SIS病例中进行评估以确定（1）是否可使用组织形态计量学鉴定损伤的证据，（2）与对照和远端切除边缘相比，近端切除边缘中ISC生物标志物表达降低，和（3）ISC生物标志物的表达与术前风险因素的数量相关，这些因素与结果、术后并发症或病例outcome.Study designRetrospective cohort study.MethodsIntestinal样本在手术中从患有SIS的成年马和因与绞痛无关的原因而被安乐死的马的切除边缘获得。从病历中获得与结局、术后并发症和病例结局呈负相关的术前风险因素。将马分组为术中、术后安乐死或存活至出院。进行组织形态计量学和免疫荧光以评估组织结构和ISC以及祖细胞数量。使用单因素ANOVA比较各组。使用线性回归模型确定生物标志物表达与术前风险因素和术后并发症数量之间的相关性，这些因素与预后呈负相关。Ki 67+细胞计数在近端（平均值= 15.45个细胞; 95% CI = 10.27-20.63; SD = 4.17;p= 0.02）和远端切缘（平均值= 15.05; 95% CI = 8.46-21.64; SD = 4.141;p= 0.03）（平均值= 23.62个细胞; 95% CI = 19.42-27.83; SD = 5.883）。在远端切除边缘，SOX 9 + Ki 67+细胞的增加与术前风险因素总数的减少相关，这些因素与预后呈负相关（95%CI = 0.236-1.123;p= 0.008，SE = 0.1393）。
BackgroundIntestinal epithelial stem cells (ISC) are responsible for epithelial regeneration and are critical to the intestine's ability to regain barrier function following injury. Evaluating ISC biomarker expression in cases of small intestinal strangulation (SIS) may provide insight into clinical progression.ObjectivesIntestinal resection margins from cases of SIS were evaluated to determine if (1) evidence of injury could be identified using histomorphometry, (2) ISC biomarker expression was decreased in the proximal resection margin compared to control and distal resection margin, and (3) the ISC biomarker expression was associated with the number of preoperative risk factors negatively related to outcome, post‐operative complications, or case outcome.Study designRetrospective cohort study.MethodsIntestinal samples were obtained intraoperatively from resection margins of adult horses with SIS and horses euthanised for reasons unrelated to colic. Preoperative risk factors negatively related to outcome, post‐operative complications, and case outcome were obtained from medical records. Horses were grouped as euthanised intraoperatively, postoperatively, or survived to discharge. Histomorphometry and immunofluorescence were performed to evaluate tissue architecture and ISC and progenitor cell number. Groups were compared using one‐way ANOVA. Associations between biomarker expression and the number of preoperative risk factors and post‐operative complications negatively related to outcome were determined using linear regression modelling.ResultsThirty‐six cases of SIS were evaluated. Ki67+cell counts were decreased in the proximal (mean = 15.45 cells; 95% CI = 10.27–20.63; SD = 4.17;p= 0.02) and distal resection margins (mean = 15.05; 95% CI = 8.46–21.64; SD = 4.141;p= 0.03) in horses euthanised postoperatively compared to control (mean = 23.62 cells; 95% CI = 19.42–27.83; SD = 5.883). In the distal resection margin, an increase in SOX9+Ki67+cells were associated with a decrease in the total number of preoperative risk factors negatively related to outcome (95% CI = 0.236–1.123;p= 0.008, SE = 0.1393).Main limitationsSmall population size.ConclusionsProliferating cell and ISC numbers may be associated with case outcome.