Snake venom causes apoptosis by increasing the reactive oxygen species in colorectal and breast cancer cell lines.
Snake venom causes apoptosis by increasing the reactive oxygen species in colorectal and breast cancer cell lines.
复制标题
蛇毒通过增加结直肠癌和乳腺癌细胞系中的活性氧而引起凋亡。
DOI：
10.2147/ott.s115055
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发表时间：
2016
影响因子：
4
通讯作者：
Islam M
中科院分区：
文献类型：
--
作者：
Al-Asmari AK;Riyasdeen A;Al-Shahrani MH;Islam M
蛇毒含有多种蛋白质和神经毒性多肽，可对神经递质信号级联产生负向干扰。这种现象的发生主要是由于体内离子通道的阻塞。中毒阻止或严重中断神经冲动的传递，抑制三磷酸腺苷的合成和心肌的正常功能。然而，毒液的一些有益特性也被报道过。本研究的目的是研究蛇毒作为抗癌剂的作用，因为它对癌症的进展有抑制作用，如细胞运动、细胞入侵和集落形成。本研究探讨了毒液对结直肠癌和乳腺癌细胞系表型变化和分子水平变化的影响。当这些细胞系用不同浓度的蛇毒处理时，观察到细胞活力、菌落形成和细胞侵袭减少了60%-90%。此外，氧化应激的增加导致凋亡癌细胞数量的增加，在毒液处理的细胞系中明显更高。进一步的分析表明，促炎细胞因子和信号蛋白的表达减少，强烈表明蛇毒在对抗乳腺癌和结直肠癌细胞进展方面有很好的作用。综上所述，本研究中使用的蛇毒对结直肠癌和乳腺癌细胞系具有显著的抗癌作用。
Snake venom possesses various kinds of proteins and neurotoxic polypeptides, which can negatively interfere with the neurotransmitter signaling cascade. This phenomenon occurs mainly due to the blocking of ion channels in the body system. Envenomation prevents or severely interrupts nerve impulses from being transmitted, inhibition of adenosine triphosphate synthesis, and proper functioning of the cardiac muscles. However, some beneficial properties of venoms have also been reported. The aim of this study was to examine the snake venom as an anticancer agent due to its inhibitory effects on cancer progression such as cell motility, cell invasion, and colony formation. In this study, the effect of venoms on phenotypic changes and the change on molecular level in colorectal and breast cancer cell lines were examined. A reduction of 60%–90% in cell motility, colony formation, and cell invasion was observed when these cell lines were treated with different concentrations of snake venom. In addition, the increase in oxidative stress that results in an increase in the number of apoptotic cancer cells was significantly higher in the venom-treated cell lines. Further analysis showed that there was a decrease in the expression of pro-inflammatory cytokines and signaling proteins, strongly suggesting a promising role for snake venom against breast and colorectal cancer cell progression. In conclusion, the snake venoms used in this study showed significant anticancer properties against colorectal and breast cancer cell lines.