A novel intracellularly expressed NS5B-specific nanobody suppresses bovine viral diarrhea virus replication
A novel intracellularly expressed NS5B-specific nanobody suppresses bovine viral diarrhea virus replication
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DOI：
10.1016/j.vetmic.2019.108449
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发表时间：
2020-01-01
影响因子：
3.3
通讯作者：
Xiao, Shuqi
中科院分区：
文献类型：
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作者：
Duan, Hong;Ma, Zhiqian;Xiao, Shuqi
牛病毒性腹泻病毒（BVDV）感染给世界范围内的养牛业造成了重大的经济损失，并且仍然对农业生产造成巨大的压力。因此，迫切需要开发新的抗BVDV策略。BVDV的非结构蛋白5（NS5B）是病毒复制所必需的。此外，骆驼单结构域抗体（纳米抗体）代表了一种有前途的抗病毒方法，具有尺寸小、结构稳定、高特异性和溶解性以及识别特异性表位的优点。然而，尚未报道抗BVDV的NS5B特异性纳米抗体。在这项研究中，NS5B特异性纳米抗体分离自山茶科重链抗体（VHH）可变结构域的噬菌体展示文库。此外，建立了稳定表达Nb 1的MDBK细胞系以探索抗病毒活性。结果表明，Nb1能显著抑制BVDV的复制，并能与BVDV NS5B蛋白相互作用。这表明纳米抗体具有开发针对BVDV感染的新型抗病毒药物的潜力。
Bovine viral diarrhea virus (BVDV) infection causes significant economic losses to the cattle industry worldwide and still represents a huge pressure on agricultural production. Thus, the development of novel anti-BVDV strategies are urgently needed. The nonstructural protein 5 (NS5B) of BVDV is essential for viral replication. Further, the camel single-domain antibody (nanobody) represents a promising antiviral approach with the advantages of small size, stable structure, high specificity and solubility, and the recognition of specific epitopes. However, no NS5B-specific nanobodies against BVDV have been reported. In this study, NS5B-specific nanobodies were isolated from a phage display library of variable domains of Camellidae heavy chain-only antibodies (VHHs). Further, an MDBK cell line stably expressing Nb1 was established to explore antiviral activity. Results showed that Nb1 could markedly suppress BVDV replication and interact with the BVDV NS5B protein. This suggests that nanobodies have potential for the development of novel antiviral drugs against BVDV infection.