Effect of pH, temperature and surface contact on the elaboration of fimbriae and flagella by Salmonella serotype Enteritidis
Effect of pH, temperature and surface contact on the elaboration of fimbriae and flagella by Salmonella serotype Enteritidis
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DOI：
10.1099/00222615-48-3-253
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发表时间：
1999-03-01
影响因子：
3
通讯作者：
Woodward, MJ
中科院分区：
文献类型：
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作者：
Walker, SL;Sojka, M;Woodward, MJ
暴露于各种环境胁迫下的肠道病原体的存活取决于许多保护性反应，其中一些与毒力决定因素的诱导有关。鞭毛和菌毛是沙门氏菌属的假定毒力决定因素，并且使用特异性检测鞭毛和 SEF21、SEF14 和 SEF17 菌毛的 ELISA 来评估温度和 pH 值对肠炎沙门氏菌血清型分离株在浮游生长中和二维梯度琼脂板表面上的加工的影响，对于肠炎沙门氏菌的三种噬菌体 4 型分离株通过比较临床来源，观察到这些表面抗原的阐述具有相似的表型。 SEF14 菌毛在 37 摄氏度（但不是 20 摄氏度）、pH 4.77 及以上（但不是 pH 4.04）的浮游生长中得到精心设计；而在琼脂梯度平板上，SEF14菌毛的精加工效果较差，但在pH 4.04时产量最高，而SEF17菌毛则在20摄氏度（但不是在37摄氏度）、pH 6.18及以上但不在pH 5.09或以下的浮游生长中精加工；而在琼脂梯度平板上，即使在 pH 4.65 下，SEF17 菌毛也能很好地表达，SEF21 菌毛在所有测试条件下的表达都非常差，37 摄氏度下的浮游生长诱导了最少的鞭毛，而 20 摄氏度下的生长，特别是较低 pH 值下的表面生长，诱导了“超鞭毛”表型。允许在梯度琼脂平板上形成的单菌落显示出产生不同的菌落形态，这些形态取决于于初始 pH 值。这些结果表明，理化环境是细菌反应的重要决定因素，尤其是假定毒力因子的诱导。
Survival of enteric pathogens exposed to various environmental stresses depends upon a number of protective responses, some of which are associated with induction of virulence determinants. Flagella and fimbriae are putative virulence determinants of Salmonella spp, and ELISAs specific for the detection of flagella and SEF21, SEF14 and SEF17 fimbriae were used to assess the effect of temperature and pH upon their elaboration by isolates of Salmonella serotype Enteritidis in planktonic growth and on the surface of two-dimensional gradient agar plates, For three phage type 4 isolates of Enteritidis of comparative clinical provenance, similar phenotypes for the elaboration of these surface antigens were observed. SEF14 fimbriae were elaborated in planktonic growth at 37 degrees C, but not 20 degrees C, at pH 4.77 and above but not at pH 4.04; whereas on agar gradient plates SEF14 fimbriae were elaborated poorly but with best yields at pH 4.04, SEF17 fimbriae were elaborated in planktonic growth at 20 degrees C, but not at 37 degrees C, at pH 6.18 and above but not at pH 5.09 or below; whereas on agar gradient plates SEF17 fimbriae were elaborated well even at pH 4.65, SEF21 fimbriae were expressed very poorly under all conditions tested, Planktonic growth at 37 degrees C induced least flagella whereas growth at 20 degrees C, and particularly surface growth at lower pH values, induced a 'hyper-flagellate' phenotype, Single colonies allowed to form on gradient agar plates were shown to generate different colonial morphologies which were dependent on initial pH. These results demonstrate that the physicochemical environment is an important determinant of bacterial response, especially the induction of putative virulence factors.