Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development.
Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development.
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DOI：
10.1111/asj.12538
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发表时间：
2016-03
期刊：
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通讯作者：
Hiroaki Nagatomo;Nanami Kohri;H. Akizawa;Y. Hoshino;N. Yamauchi;T. Kono;Masashi Takahashi;M. Kawahara
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文献类型：
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作者：
Hiroaki Nagatomo;Nanami Kohri;H. Akizawa;Y. Hoshino;N. Yamauchi;T. Kono;Masashi Takahashi;M. Kawahara
核自身抗原精子蛋白（NASP）通过与组蛋白特异性结合，与DNA复制、细胞增殖和细胞周期进程相关。本研究旨在探讨NASP在牛附植前胚胎发育中的作用。利用NASP基因敲低（KD），我们证实了NASP信使RNA（mRNA）表达的减少在植入前发育。NASP KD不影响卵裂，但显着降低胚胎发育到囊胚期。此外，NASP KD胚胎的囊胚孵化率显著降低。与对照组相比，NASP KD囊胚内细胞团中的细胞数量也减少。这些结果表明，NASP mRNA的表达所需的植入前发育到囊胚阶段的牛。
Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones. The aim of this study was to examine the roles of NASP in bovine preimplantation embryonic development. Using NASP gene knockdown (KD), we confirmed the reduction of NASP messenger RNA (mRNA) expression during preimplantation development. NASP KD did not affect cleavage but significantly decreased development of embryos into the blastocyst stage. Furthermore, blastocyst hatching was significantly decreased in NASP KD embryos. Cell numbers in the inner cell mass of NASP KD blastocysts were also decreased compared to those of controls. These results suggest that NASP mRNA expression is required for preimplantation development into the blastocyst stage in cattle.