Cell type-specific and common characteristics of exosomes derived from mouse cell lines: Yield, physicochemical properties, and pharmacokinetics
Cell type-specific and common characteristics of exosomes derived from mouse cell lines: Yield, physicochemical properties, and pharmacokinetics
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DOI：
10.1016/j.ejps.2016.10.009
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发表时间：
2017-01-01
影响因子：
4.6
通讯作者：
Takakura, Yoshinobu
中科院分区：
文献类型：
--
作者：
Charoenviriyakul, Chonlada;Takahashi, Yuki;Takakura, Yoshinobu
外泌体是细胞分泌的小膜囊泡，有望用作药物输送系统。外泌体的重要特性，如产量、理化性质和药代动力学，在不同的细胞类型中可能是不同的。然而，关于细胞类型对这些特征的影响的信息有限。在本研究中，我们评估了来自五种不同类型小鼠细胞系的外泌体的这些特征：B16BL6小鼠黑色素瘤细胞、C2C12小鼠成肌细胞、NIH3T3小鼠成纤维细胞、MAEC小鼠主动脉内皮细胞和RAW264.7小鼠巨噬细胞样细胞。外泌体采用差示超离心法收集。从所有细胞类型中收集的外泌体为带负电荷的球状囊泡，直径约为100 nm。C2C12和RAW264.7细胞比其他类型的细胞产生更多的外泌体。用高斯荧光素酶和乳粘附素的融合蛋白标记外泌体，以评估其药代动力学。经小鼠静脉注射后，所有外泌体迅速从体循环中消失，主要分布于肝脏。综上所述，不同细胞类型的外泌体产量有显著差异，本研究评估的所有外泌体均具有相似的物理化学和药代动力学性质。(C) 2016 Elsevier B.V.版权所有
Exosomes are small membrane vesicles secreted from cells and are expected to be used as drug delivery systems. Important characteristics of exosomes, such as yield, physicochemical properties, and pharmacokinetics, may be different among different cell types. However, there is limited information about the effect of cell type on these characteristics. In the present study, we evaluated these characteristics of exosomes derived from five different types of mouse cell lines: B16BL6 murine melanoma cells, C2C12 murine myoblast cells, NIH3T3 murine fibroblasts cells, MAEC murine aortic endothelial cells, and RAW264.7 murine macrophage-like cells. Exosomes were collected using a differential ultracentrifugation method. The exosomes collected from all the cell types were negatively charged globular vesicles with a diameter of approximately 100 nm. C2C12 and RAW264.7 cells produced more exosomes than the other types of cells. The exosomes were labeled with a fusion protein of Gaussia luciferase and lactadherin to evaluate their pharmacokinetics. After intravenous injection into mice, all the exosomes rapidly disappeared from the systemic circulation and mainly distributed to the liver. In conclusion, the exosome yield was significantly different among the cell types, and all the exosomes evaluated in this study showed comparable physicochemical and pharmacokinetic properties. (C) 2016 Elsevier B.V. All rights reserved.