Effects of ethanol on human lymphocyte levels of cyclic AMP. In vitro: Potentiation of the response to isoproterenol, prostaglandin E2 or adenosine stimulation.
Effects of ethanol on human lymphocyte levels of cyclic AMP. In vitro: Potentiation of the response to isoproterenol, prostaglandin E2 or adenosine stimulation.
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乙醇对人淋巴细胞环磷酸腺苷水平的影响。
DOI：
10.1111/j.1600-0773.1980.tb02026.x
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
B. Fredholm
中科院分区：
文献类型：
--
作者：
S. Hynie;F. Lanefelt;B. Fredholm
本文研究了乙醇、丙酮和二甲基亚砜（DMSO）对体外培养的人外周血淋巴细胞环腺苷酸（cAMP）蓄积的影响。异丙肾上腺素（1.0 × 10（-8）-1.0 × 10（-4）M）、PGE 2（3 × 10（-8）-3 × 10（-6）M）、腺苷（10（-7）- 10（-4）M）和苯基异丙基腺苷（10（-8）- 10（-4）M）引起环AMP蓄积的剂量依赖性增加。在整个浓度范围内的刺激药物，乙醇引起的环AMP的积累增强。在15度和30度之间的温度下，乙醇的效果随着浓度从0.2%到6%的增加而增加。在37 ℃和40 ℃下，6%乙醇的刺激作用低于2%乙醇。乙醇的影响是由丙酮和DMSO在较小程度上共享，并且也存在时，磷酸二酯酶被异丁基甲基黄嘌呤抑制。这表明，乙醇增强腺苷酸环化酶的活性作为改变细胞膜流动性的结果。由于在相当低浓度的溶剂中已经观察到对环AMP蓄积的影响，因此可能具有毒理学意义。
The effects of ethanol, acetone and dimethylsulfoxide (DMSO) were tested on the accumulation of cyclic AMP in human peripheral blood lymphocytes in vitro. Isoproterenol (1.0X10(-8) - 1.0X10(-4)M), PGE2 (3X10(-8)-3X10(-6)M), adenosine (10(-7) - 10(-4)M) and phenylisopropyladenosine (10(-8) - 10(-4)M) caused a dose dependent increase in cyclic AMP accumulation. Over the entire range of concentration of stimulating drugs, ethanol caused an enhanced accumulation of cyclic AMP. At temperatures between 15 degrees and 30 degrees the effect of ethanol rose with increasing concentration from 0.2-6%. At 37 degrees and 40 degrees, 6% ethanol had less stimulatory effect than 2% ethanol. The effect of ethanol was shared by acetone and to a minor extent by DMSO, and was present also when phosphodiesterase was inhibited by isobutylmethylxanthine. It is suggested that ethanol enhances adenylate cyclase activity as a consequence of altered cell membrane fluidity. Since the effects on cyclic AMP accumulation can be observed already at rather low concentration of the solvents they may be of toxicological significance.