Late acquisition of a germ line antibody specificity
Late acquisition of a germ line antibody specificity
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种系抗体特异性的晚期获得
作者：
N. Sigal;P. Gearhart;J. L. Press;N. Klinman
对大多数抗原决定簇的体液免疫应答的特征在于来源于大量前体细胞克隆型的高度异质性抗体分子群体1 -3。然而，对某些抗原决定簇的反应相对有限，并且可能由单一同质抗体种类主导。在近交系小鼠中，这种显性抗体特异性存在于品系4 -9的所有个体中，其特征在于相同的独特型决定子4 -8，并与重链同种异型标记7 -10相关联。因此，这些克隆型被认为是生殖系中存在的遗传信息的直接反映。被认为是“种系”的抗体特异性的一个很好的例子是BALB/c小鼠中对磷酸胆碱有反应的主要克隆型，其在其结合区域与TEPC 15和SI 07浆细胞瘤蛋白4 -5相同。在常规饲养的和无菌的BALB/c小鼠中，均发现了产生这种磷酸胆碱特异性抗体的细胞克隆的代表性高频率（19±6/106个骨髓来源的抗体形成细胞前体（B细胞））11。
THE humoral immune response to most antigenic determinants is characterised by a highly heterogeneous population of antibody molecules derived from a large array of precursor cell clonotypes1–3. The response to certain antigenic determinants, however, is relatively restricted and may be dominated by a single homogeneous antibody species. In inbred strains of mice, such dominant antibody specificities are present in all individuals of a strain4–9, are characterised by identical idiotypic determinants4–8, and are linked to heavy chain allotype markers7–10. Thus, these clonotypes are presumed to be a direct reflection of genetic information present in the germ line. A good example of an antibody specificity considered to be ‘germ line’ is the major clonotype responsive to phosphorylcholine in BALB/c mice, which is identical in its combining region to the TEPC 15 and SI07 plasmacytoma proteins4–5. Representatives of the cell clone producing this phosphoryilcholme-specific antibody have been found in high frequency (19±6 per 106 bone marrow-derived antibody-forming cell precursors (B cells)) in both conventionally reared and germ-free BALB/c mice11.