The impact of cell-specific absorption properties on the correlation of electron transport rates measured by chlorophyll fluorescence and photosynthetic oxygen production in planktonic algae
The impact of cell-specific absorption properties on the correlation of electron transport rates measured by chlorophyll fluorescence and photosynthetic oxygen production in planktonic algae
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DOI：
10.1016/j.plaphy.2011.04.010
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发表时间：
2011-08-01
影响因子：
6.5
通讯作者：
Wilhelm, Christian
中科院分区：
文献类型：
--
作者：
Blache, Ulrich;Jakob, Torsten;Wilhelm, Christian
光合作用辐射（P-E）曲线描述了自养生物的光合性能。从这些P-E-曲线可以推导出光合参数α-斜率、P-max和E-k，这些参数通常用于表征和比较不同生物或适应不同环境条件的生物。特别是对于浮游植物的P-E曲线的原位测量，可变叶绿素荧光分析证明了它作为一种灵敏和快速的方法的潜力。以普通小球藻（Trebouxiophyceae）、桑拿微拟球藻（Eustigmatophyceae）、中肋骨条藻（Spectonema costatum）和小环藻（Cyclotella meneghiniana）为材料，研究了细胞生物光学特性对荧光P-E-曲线法测定的光合参数与放氧法测定的光合参数相关性的影响。结果表明，小型浮游藻类具有广泛的细胞吸收能力，这种吸收能力受到种属特异性、生长阶段和环境条件（如营养限制）的影响。这种生物光学性质的变化导致了相对电子传递速率（rETRs）从基于氧的光合速率的很大偏差。因此，来自rETRs的光合参数α-斜率和P-max强烈依赖于特定的细胞吸收率，不能用于比较具有不同光学特性的细胞的光合性能。然而，它表明，E-k是独立的细胞吸收率，并可用于比较样品与未知的光学性能。(C)2011年Elsevier Masson SAS。All rights reserved.
Photosynthesis irradiance (P-E)-curves describe the photosynthetic performance of autotrophic organisms. From these P-E-curves the photosynthetic parameters alpha-slope, P-max, and E-k Can be deduced which are often used to characterize and to compare different organisms or organisms in acclimation to different environmental conditions. Particularly, for in situ-measurements of P-E curves of phytoplankton the analysis of variable chlorophyll fluorescence proved its potential as a sensitive and rapid method. By using Chlorella vulgaris (Trebouxiophyceae), Nannochloropsis sauna (Eustigmatophyceae), Skeletonema costatum and Cyclotella meneghiniana (Bacillariophyceae), the present study investigated the influence of cellular bio-optical properties on the correlation of the photosynthetic parameters derived from fluorescence-based P-E-curves with photosynthetic parameters obtained from the measurement of oxygen evolution. It is demonstrated that small planktonic algae show a wide range of cellular absorptivity which was subject to species-specifity, growth stage and environmental conditions, e.g. nutrient limitation. This variability in bio-optical properties resulted in a great deviation of relative electron transport rates (rETRs) from oxygen-based photosynthesis rates. Thus, the photosynthetic parameters alpha-slope and P-max derived from rETRs strongly depend on the specific cellular absorptivity and cannot be used to compare the photosynthetic performance of cells with different optical properties. However, it was shown that E-k is independent of cellular absorptivity and could be used to compare samples with unknown optical properties. (C) 2011 Elsevier Masson SAS. All rights reserved.