Magnetic spin imaging under ambient conditions with sub-cellular resolution
Magnetic spin imaging under ambient conditions with sub-cellular resolution
复制标题
DOI：
10.1038/ncomms2588
复制
发表时间：
2013-03-01
影响因子：
16.6
通讯作者：
Wrachtrup, J.
中科院分区：
文献类型：
--
作者：
Steinert, S.;Ziem, F.;Wrachtrup, J.
少量磁自旋的探测在生命、物理和化学科学中是一个重大的挑战，特别是在需要室温操作的情况下。在这里，我们展示了一个近端的氮-空位自旋系综作为高精度的传感和成像阵列。通过监测其纵向松弛，可以在不施加外部磁场的情况下检测微流控设备中自由扩散、不受干扰的磁性离子和分子。多路复用电荷耦合器件采集和优化的检测方案允许在环境条件下对磁性标记的蜂窝结构进行直接自旋噪声成像。在20个S内，我们实现了低于500 nm的空间分辨率和实验灵敏度到1000个统计极化自旋，其中只有32个离子贡献了一个净磁化强度。这些结果标志着朝着多功能亚细胞磁成像和生理条件下的实时自旋传感迈出了重要的一步，提供了一种监测活细胞内离子通道或血红蛋白运输的微创工具。
The detection of small numbers of magnetic spins is a significant challenge in the life, physical and chemical sciences, especially when room temperature operation is required. Here we show that a proximal nitrogen-vacancy spin ensemble serves as a high precision sensing and imaging array. Monitoring its longitudinal relaxation enables sensing of freely diffusing, unperturbed magnetic ions and molecules in a microfluidic device without applying external magnetic fields. Multiplexed charge-coupled device acquisition and an optimized detection scheme permits direct spin noise imaging of magnetically labelled cellular structures under ambient conditions. Within 20 s we achieve spatial resolutions below 500 nm and experimental sensitivities down to 1,000 statistically polarized spins, of which only 32 ions contribute to a net magnetization. The results mark a major step towards versatile subcellular magnetic imaging and real-time spin sensing under physiological conditions providing a minimally invasive tool to monitor ion channels or haemoglobin trafficking inside live cells.