Longitudinal near-infrared imaging of myelination.
Longitudinal near-infrared imaging of myelination.
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DOI：
10.1523/jneurosci.2698-10.2011
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发表时间：
2011-02-16
期刊：
影响因子：
--
通讯作者：
Wang Y
中科院分区：
文献类型：
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作者：
Wang C;Wu C;Popescu DC;Zhu J;Macklin WB;Miller RH;Wang Y
髓鞘形成是脊椎动物神经系统发育的基本生物学过程之一。髓鞘形成障碍被发现与许多神经系统疾病如多发性硬化症（MS）的进展有关。已经做出了巨大的努力来开发预防脱髓鞘和/或促进髓鞘再生的新型治疗剂。这些努力需要伴随着成像工具的发展，允许直接量化体内髓鞘形成。在这项工作中，我们描述了一种新的近红外荧光成像技术，能够直接量化体内髓鞘形成。该技术是基于近红外荧光探针3，3′-二乙基硫代三碳菁碘化物（DBT）开发的，该探针易于进入大脑并特异性结合有髓纤维。体内成像研究首先在两种动物模型中进行髓鞘形成过度和髓鞘形成不足，然后在铜唑酮诱导的脱髓鞘/髓鞘再生小鼠模型中进行纵向研究。定量分析表明，DBT-NIR是一种敏感和特异的髓鞘形成的成像标记物，它补充了目前其他髓鞘成像方式，并且成本低。
Myelination is one of the fundamental biological processes in the development of vertebrate nervous system. Disturbance of myelination is found to be associated with progression in many neurological diseases such as multiple sclerosis (MS). Tremendous efforts have been made to develop novel therapeutic agents that prevent demyelination and/or promote remyelination. These efforts need to be accompanied by the development of imaging tools that permit direct quantification of myelination in vivo. In this work, we describe a novel near-infrared fluorescence imaging technique that is capable of direct quantification of myelination in vivo. This technique is developed based on a near-infrared fluorescent probe, 3, 3′-Diethylthiatricarbocyanine iodide (DBT) that readily enters the brain and specifically binds to myelinated fibers. In vivo imaging studies were first conducted in two animal models of hypermyelination and hypomyelination followed by longitudinal studies in the cuprizone-induced demyelination/remyelination mouse model. Quantitative analysis suggests that DBT-NIR is a sensitive and specific imaging marker of myelination, which complements other current myelin-imaging modalities and is of low cost.