Cytokine profile of natural killer cells in early human pregnancy
Cytokine profile of natural killer cells in early human pregnancy
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DOI：
10.1111/j.1600-0897.2005.00279.x
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发表时间：
2005-07-01
影响因子：
3.6
通讯作者：
Saito, S
中科院分区：
文献类型：
--
作者：
Higuma-Myojo, S;Sasaki, Y;Saito, S
研究方法：从健康未孕妇女(n=17)、早孕妇女(6-12周，n=17)和流产病例(6-11周，n=10)外周血和/或蜕膜中分离单个核细胞。用流式细胞术检测外周血中转化生长因子β、白介素4、白介素5、白介素10、白介素13、干扰素γ和肿瘤坏死因子α的产生，以及蜕膜CD56(亮型)和CD56(Dim)NK细胞的产生。早孕妇女外周血CD56(明亮)NK细胞和CD56(Dim)NK细胞中产生IL-10的NKr1类细胞数量明显多于非妊娠妇女，流产后这些细胞数量减少。在早孕蜕膜组织中，CD56(亮)和CD56(暗)NK细胞主要是产生转化生长因子-γ的NK3型细胞，NK1型细胞很少。流产组蜕膜中NK3型细胞较正常妊娠组明显减少。结论：这些数据支持NK1/NK2/NK3/NKr1假说。外周血中的NKr1型细胞和蜕膜中的NK3型细胞可能通过调节母体免疫功能在维持妊娠中发挥重要作用。
PROBLEM: To examine whether the NK1/NK2/NK3/NKr1 paradigm can be adapted in natural killer (NK) cells.METHOD OF STUDY: Mononuclear cells were isolated from peripheral blood and/or decidua in healthy non-pregnant women (n = 17), early pregnant women (6-12) weeks of gestation, n = 17) and miscarriage cases (6-11 weeks of gestation, n = 10). We investigated the production of transforming growth factor (TGF)-beta, interleukin (IL)-4, IL-5, IL-10, IL-13, interferon (IFN)-gamma and tumor necrosis factor-alpha from peripheral blood- and decidual-CD56(bright) NK cells and -CD56(dim) NK cells by flow cytometry.RESULTS: In the peripheral blood of the non-pregnant subjects, the main populations of CD56(bright) NK cells and CD56(dim) NK cells were IFN-gamma-producing NK 1 type cells. Populations of IL-10-producing NKr1 type cells in peripheral blood CD56(bright) NK cells and CD56(dim) NK cells in early pregnant women were significantly greater compared with those in non-pregnant women, and these cells population decreased in miscarriage cases. In the early pregnancy decidua, the main populations of CD56(bright) NK cells and CD56(dim) NK cells were TGF-gamma-producing NK3 type cells, and NK1 type cells were rare. NK3 type cells in decidua were significantly decreased in miscarriage cases compared with those in normal pregnant subjects. IL-4-, IL-5- or IL-13-producing NK2 type cells were rare in peripheral blood and decidua.CONCLUSION: These data support the NK1/NK2/NK3/NKr1 hypothesis. NKr1 type cells in peripheral blood and NK3 type cells in decidua might play some important roles in the maintenance of pregnancy by regulation of maternal immune function.