CELL-PROLIFERATION WITHIN BOWMAN CAPSULE IN MICE
CELL-PROLIFERATION WITHIN BOWMAN CAPSULE IN MICE
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DOI：
10.1002/path.1711450405
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发表时间：
1985-01-01
影响因子：
7.3
通讯作者：
WHEELER, J
中科院分区：
文献类型：
--
作者：
MORLEY, AR;WHEELER, J
新月体肾小球肾炎，无论病因如何，提示严重的肾小球损害，预后不良。巨噬细胞在肾小球新月体发育中的重要性已得到充分证实，但巨噬细胞和上皮细胞中细胞增殖的作用却鲜有人关注。建立了一种适合细胞动力学研究的小鼠新月体肾炎模型。本文介绍了兔抗小鼠肾小球基底膜(GBM)抗血清免疫的初步研究，包括用兔免疫球蛋白预免后，用纤溶抑制物处理和不用处理。Cyklokapron。每日静脉注射4次，可造成广泛的肾小球损害，并伴有新月体形成。用兔免疫球蛋白预免后注射兔抗小鼠GBM抗血清。这种影响是剂量依赖的，在最后一次注射后3天内出现。免疫荧光法检测到兔免疫球蛋白呈线状沉积，小鼠免疫球蛋白呈线状和颗粒状沉积。包曼S胶囊组肾小球大小和细胞数显著增加(P<；0.01)，有丝分裂指数在第13天增加到0.8%。
Crescentic glomerulonephritis in man, irrespective of etiology, indicates severe glomerular damage and carries a poor prognosis. The importance of the presence of macrophages in the development of glomerular crescents is well established, but the contribution of cellular proliferation in both macrophages and epithelial cells has received little attention. A model of crescentic glomerulonephritis was developed in mice, which is suitable for cell kinetic studies. Preliminary studies are described, involving immunization with rabbit anti-mouse glomerular basement membrane (GBM) antiserum after pre-immunization with rabbit IgG, both with and without treatment with a fibrinolytic inhibitor. Cyklokapron. Extensive glomerular damage, with crescent formation, was induced with 4 daily i.v. injections of rabbit anti-mouse GBM antiserum, after pre-immunization with rabbit IgG. The effect was dose dependant and was seen within 3 days of the last injection. Linear deposits of rabbit IgG were detected in all glomeruli by immunofluorescence, and both linear and granular deposits of mouse IgG were also found. There was a significant increase in glomerular size and in the numbers of cells in Bowman''s capsule (P < 0.01), and increases in mitotic indices to 0.8% were seen by day 13.