Pan-neuronal knockdown of the c-Jun N-terminal Kinase (JNK) results in a reduction in sleep and longevity in Drosophila
Pan-neuronal knockdown of the c-Jun N-terminal Kinase (JNK) results in a reduction in sleep and longevity in Drosophila
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DOI：
10.1016/j.bbrc.2011.12.040
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发表时间：
2012-01-13
影响因子：
3.1
通讯作者：
Kume, Kazuhiko
中科院分区：
文献类型：
--
作者：
Takahama, Kazuhiro;Tomita, Jun;Kume, Kazuhiko
睡眠是一种独特的行为状态，在物种之间是保守的，睡眠调节与新陈代谢和衰老密切相关。果蝇（Drosophila melanogaster）被用来研究这些生理过程背后的分子机制。在这里，我们展示了c-Jun n -末端激酶（JNK）基因，在果蝇中被称为篮子（bsk），在神经元中起作用，调节果蝇的睡眠和寿命。通过RNA干扰抑制JNK mRNA的泛神经元表达导致睡眠和寿命减少。杂合敲除JNK显示出类似的效果，表明分子特异性。JNK敲低显示了正常的唤醒阈值和睡眠反弹，这表明基本的睡眠机制没有受到影响。已知JNK参与胰岛素通路，调节新陈代谢和寿命。在脑间部产生胰岛素的神经元中，JNK的下调对睡眠有轻微的影响。然而，敲除蘑菇体内的INK对睡眠有显著影响。这些数据表明INK有一种独特的睡眠调节途径。(C) 2011爱思唯尔公司版权所有。
Sleep is a unique behavioral state that is conserved between species, and sleep regulation is closely associated to metabolism and aging. The fruit fly, Drosophila melanogaster has been used to study the molecular mechanism underlying these physiological processes. Here we show that the c-Jun N-terminal Kinase (JNK) gene, known as basket (bsk) in Drosophila, functions in neurons to regulate both sleep and longevity in Drosophila. Pan-neuronal knockdown of JNK mRNA expression by RNA interference resulted in a decrease in both sleep and longevity. A heterozygous knockout of JNK showed similar effects, indicating the molecular specificity. The JNK knockdown showed a normal arousal threshold and sleep rebound, suggesting that the basic sleep mechanism was not affected. JNK is known to be involved in the insulin pathway, which regulates metabolism and longevity. A JNK knockdown in insulin-producing neurons in the pars intercerebralis had slight effects on sleep. However, knocking down INK in the mushroom body had a significant effect on sleep. These data suggest a unique sleep regulating pathway for INK. (C) 2011 Elsevier Inc. All rights reserved.