Functional characterization of CYP52G3 from Aspergillus oryzae and its application for bioconversion and synthesis of hydroxyl flavanone and steroids
Functional characterization of CYP52G3 from Aspergillus oryzae and its application for bioconversion and synthesis of hydroxyl flavanone and steroids
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DOI：
10.1002/bab.1496
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发表时间：
2017-05
影响因子：
2.8
通讯作者：
T. Uno;T. Yanase;H. Imaishi
中科院分区：
文献类型：
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作者：
T. Uno;T. Yanase;H. Imaishi
曲霉菌是一种广泛用于日本传统发酵工业的真菌。细胞色素P450（Cytochrome P450，CYP）蛋白广泛分布于自然界，具有多种酶活性。发现了一个新的CYP 52基因（CYP 52 G3）。- 是的在这项研究中，我们报告了CYP 52 G3的功能特性。重组蛋白在大肠杆菌中异源表达，并分离其膜组分。CYP 52 G3对7-乙氧基香豆素和α-萘黄酮有活性。此外，CYP 52 G3在4′和6位羟基化黄烷酮，并代谢一些羟基黄烷酮和类固醇。生物转化实验表明，CYP 52 G3可以在合成培养基中转化黄烷酮和睾酮。24 H时黄烷酮和睾酮的转化率分别为50%和70%。这些结果支持CYP 52 G3可以证明是一种有用的酶，用于从类黄酮和类固醇有效生产新化合物。
Aspergillus oryzae is a fungus widely used in traditional Japanese fermentation industries. Cytochrome P450 (CYP) proteins are ubiquitously distributed in nature and display a broad range of enzymatic activities. A novel CYP52 (CYP52G3) gene was found in A. oryzae. In this study, we report the functional characterization of CYP52G3. The recombinant protein was expressed heterologously in Escherichia coli, and its membrane fraction isolated. CYP52G3 showed activities for 7‐ethoxycoumarin and α‐naphtoflavone. Furthermore, CYP52G3 hydroxylated flavanone at the 4′ and 6 position and metabolized some hydroxyl‐flavanones and steroids. Bioconversion experiments indicated that CYP52G3 could convert flavanone and testosterone in a synthetic medium. The conversion rates of flavanone and testosterone at 24 H were 50% and 70%, respectively. These results support that CYP52G3 could prove a useful enzyme for the efficient production of new compounds from flavonoids and steroids.