Real-time linux dynamic clamp: A fast and flexible way to construct virtual ion channels in living cells
Real-time linux dynamic clamp: A fast and flexible way to construct virtual ion channels in living cells
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DOI：
10.1114/1.1408929
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发表时间：
2001-10-01
影响因子：
3.8
通讯作者：
White, JA
中科院分区：
文献类型：
--
作者：
Dorval, AD;Christini, DJ;White, JA
我们描述了一个用于实时控制生物和其他实验的系统。该设备基于实时Linux操作系统，专门在动态箝位的背景下进行了测试，动态箝位是一项要求严格的实时任务，其中计算系统模拟活细胞中非线性膜电导的影响。该系统是足够快，以代表几十个非线性电导在真实的时间在时钟频率远高于10 kHz。电导可以以确定性的形式表示，或者更准确地说是随机门控离子通道的离散集合。使用各种复杂的模型，在可兴奋细胞的非线性膜机制，包括模拟空间扩展的可兴奋结构和多个相互作用的细胞进行测试。仅在极端情况下计算负荷才干扰高速“硬”实时处理（即，实时处理，永不动摇）。该实验控制系统可在全球网络上免费获得，具有良好的性能、极大的灵活性、低成本和合理的易用性。它很容易适应任何涉及实时控制的任务，特别是对于需要复杂控制算法的应用，必须以超过1 kHz的速度运行。(C)2001生物医学工程学会。
We describe a system for real-time control of biological and other experiments. This device, based around the Real-Time Linux operating system, was tested specifically in the context of dynamic clamping, a demanding real-time task in which a computational system mimics the effects of nonlinear membrane conductances in living cells. The system is fast enough to represent dozens of nonlinear conductances in real time at clock rates well above 10 kHz. Conductances can be represented in deterministic form, or more accurately as discrete collections of stochastically gating ion channels. Tests were performed using a variety of complex models of nonlinear membrane mechanisms in excitable cells, including simulations of spatially extended excitable structures and multiple interacting cells. Only in extreme cases does the computational load interfere with high-speed "hard" real-time processing (i.e., real-time processing that never falters). Freely available on the worldwide web, this experimental control system combines good performance, immense flexibility, low cost, and reasonable ease of use. It is easily adapted to any task involving real-time control, and excels in particular for applications requiring complex control algorithms that must operate at speeds over 1 kHz. (C) 2001 Biomedical Engineering Society.