Augmented blood pressure variability following continuous infusion of noradrenaline in rats
Augmented blood pressure variability following continuous infusion of noradrenaline in rats
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DOI：
10.1097/hjh.0000000000002239
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发表时间：
2020-02-01
影响因子：
4.9
通讯作者：
Kato, Johji
中科院分区：
文献类型：
--
作者：
Jiang, Danfeng;Kawagoe, Yukiko;Kato, Johji
目的：血压升高（BP）变异性与心血管疾病有关。交感神经系统的活动是24小时血压变异性的重要决定因素，尽管尚不清楚持续的肾上腺素能激活是否会导致血压变异性增强。在这里，我们报告持续输注去甲肾上腺素增加大鼠24小时血压变异性。方法：9周龄雄性Wistar大鼠连续皮下注射去甲肾上腺素30 μ g/h、α - 1肾上腺素激动剂苯肾上腺素150 μ g/h或β -肾上腺素激动剂异丙肾上腺素30 μ g/h，持续14 d。输注去甲肾上腺素的大鼠在输注期间分别口服5 mg/天的吡唑嗪或50 mg/天的阿替洛尔。在不受约束的情况下，通过腹主动脉导管通过无线电遥测系统每15分钟记录一次血压变异系数，评估输注7天和14天前后的血压变异性。结果：持续输注去甲肾上腺素显著增加了第7天和第14天的24小时血压变异性，轻微升高血压水平，而这种增加的血压变异性被普唑嗪部分减弱，而阿替洛尔则没有。持续输注苯肾上腺素增加了血压变异性，而异丙肾上腺素对变异性没有影响。结论：持续输注去甲肾上腺素增强大鼠24小时血压变异性的机制部分是通过α 1-肾上腺素受体介导的机制，提示去甲肾上腺素输注大鼠是持续肾上腺素能激活引起的血压变异性增强的动物模型。
Objective: Augmented blood pressure (BP) variability has been shown to be associated with cardiovascular diseases. Activity of the sympathetic nervous system is an important determinant factor of the 24-h profile of BP variability, although it is unknown whether persistent adrenergic activation causes augmented BP variability or not. Here we report that continuous infusion of noradrenalin augments 24-h BP variability in rats. Methods: Nine-week-old male Wistar rats were continuously infused with subcutaneous 30 mu g/h noradrenalin, 150 mu g/h of the alpha 1-adrenergic agonist phenylephrine, or 30 mu g/h of the beta-agonist isoproterenol, for 14 days. Noradrenalin-infused rats were also administered either oral 5 mg/day prazosin or 50 mg/day atenolol during the infusion period. BP variability was evaluated before and after 7 and 14 days of the infusion, using a coefficient of variation of BP recorded every 15 min under an unrestrained condition via an abdominal aortic catheter by a radiotelemetry system. Results: Continuous infusion of noradrenalin significantly increased 24-h BP variability at 7 and 14 days, slightly elevating BP levels, while this increase in BP variability was partially attenuated by prazosin, but not by atenolol. Continuous infusion of phenylephrine augmented BP variability, but isoproterenol had no effect on the variability. Conclusion: Continuous infusion of noradrenalin augmented 24-h BP variability partly through an alpha 1-adrenergic receptor-mediated mechanism in rats, suggesting that the noradrenalin-infused rat is an animal model of augmented BP variability induced by persistent adrenergic activation.