A novel periplasmic protein (Slr0280) tunes photomixotrophic growth of the cyanobacterium, Synechocystis sp PCC 6803
A novel periplasmic protein (Slr0280) tunes photomixotrophic growth of the cyanobacterium, Synechocystis sp PCC 6803
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一种新型周质蛋白 (Slr0280) 调节蓝藻集胞藻 PCC 6803 的光合营养生长
DOI：
10.1016/j.gene.2015.09.015
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发表时间：
2016
期刊：
影响因子：
3.5
通讯作者：
Zhao Kai-Hong
中科院分区：
文献类型：
--
作者：
Dong Liang-Liang;Li Qing-Dong;Wu Dong;Sun Ya-Fang;Zhou Ming;Zhao Kai-Hong
蓝藻是全球光合作用的主要贡献者之一，并表现出高度的代谢可塑性。集胞藻属。 PCC 6803 可以在光合自养、光合营养或光合异养条件下生长。我们已经表征了一种新型周质蛋白（Slr0280），它可以调节集胞藻的光合营养生长。 PCC 6803。Slr0280 是一种主要由 β-折叠组成的多结构域蛋白。通过细菌双杂交筛选鉴定了几种与 Slr0280 相互作用的蛋白质。 Slr0280可能通过其DUF2233结构域与参与糖代谢的伙伴相互作用，从而协调各自的调节。当slr0280被删除时，突变体在葡萄糖存在下比野生型生长得更慢，这归因于糖酵解、糖原分解代谢、氧化戊糖磷酸途径、卡尔文循环和葡萄糖利用的下调。转录本 (qPCR) 分析证实 Slr0280 对这些糖分解代谢酶的正向调节。基于这些发现，我们提出了一个推测模型，即 Slr0280 在糖代谢中发挥协调调节作用。
Cyanobacteria are among the main contributors to global photosynthesis and show a high degree of metabolic plasticity. Synechocystis sp. PCC 6803 can grow under photoautotrophic, photomixotrophic or photoheterotrophic conditions. We have characterized a novel periplasmic protein (Slr0280) that tunes the photomixotrophic growth ofSynechocystissp. PCC 6803. Slr0280 is a multi-domain protein consisting mainly of β-sheets. Several proteins that interact with Slr0280 were identified via bacterial two-hybrid screening. Slr0280 may interact through its DUF2233 domain with partners that participate in sugar metabolism, thereby coordinating the respective regulations. Whenslr0280was deleted, the mutant grew more slowly than wild-type in the presence of glucose, which is ascribed to the down-regulation of glycolysis, glycogen catabolism, oxidative pentose phosphate pathway, Calvin cycle and glucose utilization. A positive regulation of Slr0280 on these sugar catabolic enzymes was confirmed by transcript (qPCR) analyses. Based on these findings, we proposed a speculative model that Slr0280 plays a coordinating regulatory role in sugar metabolism.