Isolation, structure and biological activity of phomafungin, a cyclic lipodepsipeptide from a widespread tropical Phoma sp.
Isolation, structure and biological activity of phomafungin, a cyclic lipodepsipeptide from a widespread tropical Phoma sp.
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DOI：
10.1016/j.bmc.2008.12.009
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发表时间：
2009-02-01
影响因子：
3.5
通讯作者：
Singh, Sheo B.
中科院分区：
文献类型：
--
作者：
Herath, Kithsiri;Harris, Guy;Singh, Sheo B.
我们从茎点霉属植物中分离出一种环状脂缩肽（lipodepsipeptide），即芬净（phomafungin）。白色念珠菌中的一种。体能测试纯化的化合物含有由8个氨基酸和β-羟基-γ-甲基-十六烷酸组成的28元环，并显示出对念珠菌属的广谱抗真菌活性，烟曲霉和须毛癣菌的MIC为2-8 μ g/ml，25 mg/kg对小鼠有毒性。内酯环开环得到的线性肽没有抗真菌活性，也没有毒性。已在许多茎点霉属中鉴定出Phomafungin。采集自非洲、印度洋和太平洋岛屿。(c)2009爱思唯尔有限公司保留所有权利。
We isolated a cyclic lipodepsipeptide, phomafungin, from a Phoma sp. The distinct antifungal activity of phomafungin in the crude extract was initially discovered by mechanistic pro. ling in the Candida albicans. fitness test. The purified compound contains a 28 member ring consisting of eight amino acids and a beta-hydroxy-gamma-methyl-hexadecanoic acid, and displays a broad spectrum of antifungal activity against Candida spp., Aspergillus fumigatus and Trichophyton mentagrophytes with MIC of 2-8 mu g/ml, and toxicity to mice at 25 mg/kg. The linear peptide derived from opening of the lactone ring was devoid of antifungal activity as well as toxicity. Phomafungin has been identified in a number of Phoma spp. collected from Africa and the Indian and Pacific Ocean islands. (c) 2009 Elsevier Ltd. All rights reserved.