Bio-electrospraying embryonic stem cells: interrogating cellular viability and pluripotency
Bio-electrospraying embryonic stem cells: interrogating cellular viability and pluripotency
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DOI：
10.1039/b819889f
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发表时间：
2009-03-01
影响因子：
2.5
通讯作者：
Jayasinghe, Suwan N.
中科院分区：
文献类型：
--
作者：
Abeyewickreme, Anil;Kwok, Albert;Jayasinghe, Suwan N.
生物电喷雾是最近发现的一种直接电场驱动的细胞工程过程，已被证明在分子水平上对处理后的细胞没有有害影响。尽管与对照组相比，来自永生化和原代培养的几种细胞类型在处理后作为时间函数进行了评估，但该方案尚未应用于胚胎干细胞。如果生物电喷雾要进一步推广其适用性，尤其是在组织工程和再生医学方面，这一点是最重要的，因为胚胎干细胞在这些领域发挥着基础作用。在这里介绍的研究中，所选择的干细胞是小鼠胚胎干细胞。因此，这些通过生物电喷雾的方式喷射胚胎干细胞的第一个例子表明，当将这些处理后的细胞与各自的对照细胞进行比较时，细胞的活性和细胞的多能性是无法区分的。
Bio-electrospraying, a recently discovered, direct electric field driven cell engineering process, has been demonstrated to have no harmful effects on treated cells at a molecular level. Although several cell types from both immortalized and primary cultures have been assessed post-treatment as a function of time in comparison to controls, the protocol has yet to be applied on embryonic stem cells. This is most important if bio-electrosprays are to further their applicability, in particular with regard to tissue engineering and regenerative medicine, where embryonic stem cells play a fundamental role. In the study presented herein the chosen stem cells are mouse embryonic stem (ES) cells. Hence, these first examples where embryonic stem cells have been jetted by way of bio-electrosprays, demonstrate the cellular viability and the cell's pluripotency indistinguishable when comparing those post-treated cells with their respective controls.