Endometrium as an early sensor of in vitro embryo manipulation technologies
Endometrium as an early sensor of in vitro embryo manipulation technologies
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DOI：
10.1073/pnas.0812722106
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发表时间：
2009-04-07
影响因子：
11.1
通讯作者：
Renard, Jean-Paul
中科院分区：
文献类型：
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作者：
Mansouri-Attia, Nadera;Sandra, Olivier;Renard, Jean-Paul
着床对胎盘发育至关重要，胎盘发育会影响胎儿生长和妊娠成功，从而影响出生后的健康。我们推测，当胚胎附着在母亲子宫上时，子宫内膜表达的基因模式可能会解释怀孕的最终结果。作为一个模型，我们使用了胚胎从怀孕第20天起逐渐永久附着于子宫内膜的牛物种。在那个阶段，我们比较了体内受精胚胎(AI)和核移植(SCNT)或体外受精胚胎(IVF)获得的子宫内膜基因图谱，两者都显示出较低的和不同的足月发育潜力。我们的数据提供的证据表明，子宫内膜可以被视为一个生物传感器，能够微调其生理功能，以应对胚胎的存在，这些胚胎的发育将在植入过程后很长一段时间内发生变化。与人工授精相比，SCNT胚胎着床时子宫内膜的许多生物学功能和几条对新陈代谢和免疫功能有重要影响的典型途径都发生了显著变化，而IVF胚胎的这种差异则不明显。在着床开始时确定子宫内膜可塑性的界限，将为母体环境对胚胎发育和妊娠成功的贡献带来新的见解。
Implantation is crucial for placental development that will subsequently impact fetal growth and pregnancy success with consequences on postnatal health. We postulated that the pattern of genes expressed by the endometrium when the embryo becomes attached to the mother uterus could account for the final outcome of a pregnancy. As a model, we used the bovine species where the embryo becomes progressively and permanently attached to the endometrium from day 20 of gestation onwards. At that stage, we compared the endometrial genes profiles in the presence of an in vivo fertilized embryo ( AI) with the endometrial patterns obtained in the presence of nuclear transfer ( SCNT) or in vitro fertilized embryos (IVF), both displaying lower and different potentials for term development. Our data provide evidence that the endometrium can be considered as a biological sensor able to fine-tune its physiology in response to the presence of embryos whose development will become altered much later after the implantation process. Compared with AI, numerous biological functions and several canonical pathways with a major impact on metabolism and immune function were found to be significantly altered in the endometrium of SCNT pregnancies at implantation, whereas the differences were less pronounced with IVF embryos. Determining the limits of the endometrial plasticity at the onset of implantation should bring new insights on the contribution of the maternal environment to the development of an embryo and the success of pregnancy.