A putative transcription factor LFC1 negatively regulates development and yield of winter mushroom
A putative transcription factor LFC1 negatively regulates development and yield of winter mushroom
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DOI：
10.1007/s00253-020-10642-8
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发表时间：
2020-04
影响因子：
5
通讯作者：
Taju Wu;Chengcheng Hu;B. Xie;Shenglong Wei;Long Zhang;Zixiong Zhu;Zhen-ying Zhang;Shaojie Li
中科院分区：
文献类型：
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作者：
Taju Wu;Chengcheng Hu;B. Xie;Shenglong Wei;Long Zhang;Zixiong Zhu;Zhen-ying Zhang;Shaojie Li
担子实体是蘑菇类的子实体。深入了解担子菌的发育机制，对蘑菇的育种和栽培具有重要的指导意义。从工厂化栽培的五大食用菌之一的冬菇中，发现了一种新的锌(II)2Cys6转录因子LFC1，该转录因子在担子实体的发育过程中具有负调控功能。在担子癌发生过程中，flc1的转录水平显著降低。Fc1的过度表达和敲除均不影响菌丝的营养生长。然而，fc1基因的敲除可以促进担子实体的发育，使培养时间缩短2天，而fc1基因的过度表达则将最佳收获期推迟3天。在fc1基因表达下调72%的菌株中，食用菌产量和生物学效率至少可提高24%。Fc1基因的敲除不影响菌帽的形状，但显著增加基节的长度和数目，而其过表达不影响基节的长度，但显著减少基节的数目。此外，与野生型不同，在1fc1高表达突变体中，担子虫的帽状突变体的帽状突变体的帽明显较大，帽缘起皱。RNA-seq分析表明，455个基因对olfc1的过度表达和基因敲除有相反的转录反应。其中包括3个疏水素编码基因、2个凝集素编码基因、Eln2同源编码基因FVFD16和3个膜成分编码基因。由于LFc1同源基因广泛存在于蘑菇中，LFc1可用于蘑菇育种。要点·一个新的转录因子LFc1负调控冬菇的结果·LFc1调控400多个基因的转录。·降低LFc1的表达可以缩短栽培时间，提高产量。·lfc1可能是一个潜在的有用的参考基因。
Basidioma is the fruiting body of mushroom species. The deep understanding on the mechanism of basidioma development is valuable for mushroom breeding and cultivation. From winter mushroom (Flammulina velutipes), one of the top five industrially cultivated mushrooms, a novel putative Zn(II)2Cys6 transcription factor LFC1 with negative regulatory function in basidioma development was identified. The transcript level oflfc1was dramatically decreased during basidioma development. Neither overexpression nor knockdown oflfc1affected hyphal vegetative growth. However, knockdown oflfc1could promote basidioma development and shorten cultivation time by 2 days, while overexpression oflfc1delayed the optimal harvest time by 3 days. In thelfc1knockdown strain, in which thelfc1expression was reduced by 72%, mushroom yield and biological efficiency could be increased at least by 24%. Knockdown oflfc1did not affect the shape of caps but significantly increased basidioma length and number, while its overexpression did not affect basidioma length but dramatically reduced basidioma number. In addition, rather than producing basidiomata with round caps as in wild type, the caps of basidiomata in thelfc1overexpression mutants were significantly larger and the cap edge was wrinkled. RNA-seq analysis revealed that 455 genes had opposite transcriptional responses tolfc1overexpression and knockdown. Some of them were previously reported as genes involved in basidioma development, including 3 hydrophobin encoding genes, 2 lectin encoding genes,FVFD16, an Eln2 ortholog encoding gene, and 3 genes encoding membrane components. As LFC1 homologs are widely present in mushroom species,lfc1can be useful in mushroom breeding.Key Points• A novel transcription factor LFC1 negatively regulates fruiting in winter mushroom• LFC1 regulated transcription of more than 400 genes.• Reduction of LFC1 expression could shorten cultivation time and increase yield.• lfc1 could be a potentially useful reference gene for mushroom breeding.