PDCD4 expression inversely correlated with miR-21 levels in gastric cancers
PDCD4 expression inversely correlated with miR-21 levels in gastric cancers
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DOI：
10.1007/s00432-011-1140-8
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发表时间：
2012-04-01
影响因子：
3.6
通讯作者：
Park, Won Sang
中科院分区：
文献类型：
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作者：
Cao, Zhang;Yoon, Jung Hwan;Park, Won Sang
本研究的具体目的是探讨PDCD4基因是否参与了胃癌的发生发展。我们检测了PDCD4基因在胃癌中的遗传和表观遗传学改变以及PDCD4蛋白的表达。用实时荧光定量RT-PCR法分析PDCD4mRNA和miRNA-21的表达，发现215例胃癌组织中有79例(36.7%)PDCD4表达降低或降低。统计学分析显示，PDCD4表达改变与肿瘤分化程度、肿瘤部位、淋巴结转移和总生存期等临床病理参数无关(P>；0.05)。胃癌组织中miRNA-21高表达的发生率为67.4%(31/46)，且miRNA-21与PDCD_4蛋白表达呈显著负相关(P=0.029)，而与PDCD_4基因表达无相关性。在基因分析中，66例胃癌组织中有24例(36.4%)未检测到PDCD4基因编码区的突变，有24例(36.4%)存在启动子高甲基化，提示miRNA-21的过度表达和PDCD4表达的降低或缺失可能在胃癌的发生发展中起一定作用。
The specific aim of this study was to investigate whether the PDCD4 gene is involved in the development and progression of gastric cancer.We examined the genetic and epigenetic alterations of the PDCD4 gene as well as the expression of PDCD4 protein in gastric cancers. The mRNA expression of PDCD4 and miRNA-21 expression were also analyzed using quantitative real-time RT-PCR.Loss or reduced PDCD4 expression was observed in 79 (36.7%) of 215 gastric cancer specimens. Statistically, altered PDCD4 expression was not associated with the clinicopathological parameters, including tumor differentiation, location, lymph node metastasis and overall survival (P > 0.05). miRNA-21 overexpression was frequently detected in gastric cancers (31 of 46, 67.4%), and there was a significant inverse correlation between miRNA-21 and PDCD4 protein expression (P = 0.029), but not between miRNA-21 and PDCD4 mRNA expression. In genetic analysis, no mutation was detected in the coding region of the PDCD4 gene, and promoter hypermethylation was found in 24 (36.4%) of the 66 gastric cancer samples.Our data suggest that overexpression of miRNA-21 and reduced or loss of PDCD4 expression may play a role in the development and progression of gastric cancers.