Tandem lesions are the major products resulting from a pyrimidine nucleobase radical
Tandem lesions are the major products resulting from a pyrimidine nucleobase radical
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DOI：
10.1021/ja036629u
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发表时间：
2003-11-05
影响因子：
15
通讯作者：
Greenberg, MM
中科院分区：
文献类型：
--
作者：
Carter, KN;Greenberg, MM
核碱基自由基（例如，1）是 DNA 暴露于 γ-放射解时形成的反应中间体的主要家族。化学合成寡核苷酸中 1 的独立生成表明，在有氧条件下这种核碱基自由基的形成大约 65% 的时间会导致串联损伤的形成。 5'-和3'-相邻核苷酸形成的损伤的分布取决于双链DNA的二级结构。串联损伤被定义为单条 DNA 链中两个连续的受损核苷酸，具有重要的生物学意义。 1 的串联损伤的产量比以前认为的要高得多。这些观察结果可能会对科学家如何解释 γ 放射解作用对 DNA 的影响产生重大影响。
Nucleobase radicals (e.g.,1) are the major family of reactive intermediates formed when DNA is exposed to γ-radiolysis. Independent generation of1in chemically synthesized oligonucleotides reveals that formation of this nucleobase radical under aerobic conditions results in the formation of tandem lesions approximately 65% of the time. The distribution of lesions formed with the 5‘- and 3‘-adjacent nucleotides is dependent upon the secondary structure of duplex DNA. Tandem lesions, which are defined as two contiguously, damaged nucleotides in a single DNA strand, are of significant biological interest. The yield of tandem lesions from1is much greater than was previously believed. The observations presented could have significant ramifications on how scientists interpret the effects of γ-radiolysis on DNA.