The production of superoxide anion and nitric oxide by cultured murine leukocytes and the accumulation of TNF-alpha in the conditioned media is inhibited by taurine chloramine
The production of superoxide anion and nitric oxide by cultured murine leukocytes and the accumulation of TNF-alpha in the conditioned media is inhibited by taurine chloramine
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DOI：
10.1016/0162-3109(96)00113-0
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发表时间：
1996-09-01
期刊：
影响因子：
--
通讯作者：
SchullerLevis, G
中科院分区：
文献类型：
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作者：
Kim, C;Park, E;SchullerLevis, G
牛磺酸氯胺（Tau-Cl）抑制活化的腹腔巨噬细胞产生一氧化氮（NO），并减弱培养基中肿瘤坏死因子-α（TNF-α）的积累，与先前报道的活化RAW 264.7细胞相似。此外，还观察了Tau-Cl和牛磺酸对小鼠腹腔渗出液多形核白细胞（PMN）产生超氧阴离子（O-2（-））的影响。Tau-Cl以剂量依赖性和可逆的方式抑制O-2（-）的产生。牛磺酸也抑制O-2（-）的生产刺激PMN，但在较高的浓度和程度低于Tau-Cl。牛磺酸对O-2（-）产生的影响归因于中性粒细胞相关的卤素依赖性髓过氧化物酶在体外催化Tau-Cl的形成。相反，激活的腹腔巨噬细胞产生NO和介质中TNF-α的积累被Tau-Cl抑制，而牛磺酸没有影响。这些数据支持了Tau-Cl可能通过调节炎症介质的产生而参与炎症反应的观点。
Taurine chloramine (Tau-Cl) inhibits production of nitric oxide (NO) by activated peritoneal macrophages and attenuates accumulation of tumor necrosis factor-alpha (TNF-a) in the culture media, similar co that previously reported for activated RAW 264.7 cells. In addition, the effect of Tau-Cl and taurine on superoxide anion (O-2(-)) production in murine peritoneal exudate polymorphonuclear leukocytes (PMN) was examined. Tau-Cl inhibited O-2(-) production in a manner that was dose-dependent and reversible. Taurine also inhibited O-2(-) production by stimulated PMN, but at higher concentrations and to a lesser extent than Tau-Cl. The effects of taurine on O-2(-) production was attributed to the in vitro formation of Tau-Cl catalyzed by PMN associated halide-dependent myeloperoxidase. In contrast, production of NO by activated peritoneal macrophages and accumulation of TNF-alpha in the media was inhibited by Tau-Cl while taurine was without effect. These data lend support to the notion that Tau-Cl may participate in the inflammatory response by modulating production of inflammatory mediators.