Molecular and functional characterization of a ladderlectin-like molecule from ayu (Plecoglossus altivelis).
Molecular and functional characterization of a ladderlectin-like molecule from ayu (Plecoglossus altivelis).
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DOI：
10.1016/j.fsi.2022.10.023
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发表时间：
2022-10
影响因子：
4.7
通讯作者：
W. Wang;Mei Liu;Chenjie Fei;Chang-hong Li;Jiong Chen
中科院分区：
文献类型：
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作者：
W. Wang;Mei Liu;Chenjie Fei;Chang-hong Li;Jiong Chen
Ladderlectin 是硬骨鱼中 C 型凝集素 (CTL) 的成员，参与先天免疫防御。在这项研究中，克隆了香鱼（Plecoglossus altivelis）梯状蛋白（PaLL-like）序列，该序列编码172个氨基酸的多肽，其中包括信号肽和特征性C型凝集素样结构域（CTLD）。从系统发育上看，PaLL-like与其来自什沙莫胡瓜鱼（Spirinchus lanceolatus）的硬骨鱼对应物关系最为密切。表达分析显示其普遍存在的表达谱，在肝脏中检测到最高表达，并且其表达在弧菌感染后上调。与典型的 CTL 类似，PaLL 样对多种明确的单糖/二糖表现出碳水化合物结合能力，并可能赋予 PaLL 样凝集所有测试细菌的能力，包括三种革兰氏阳性菌（即单核细胞增多性李斯特菌、金黄色葡萄球菌和海豚链球菌）和八种革兰氏阴性菌（即爱德华氏菌）嗜水气单胞菌、大肠杆菌、哈维氏弧菌、副溶血弧菌、创伤弧菌和创伤弧菌），以钙依赖性方式。进一步的功能研究表明，PaLL-like 表现出免疫调节活性，导致血清杀菌活性增强、病原体调理作用和巨噬细胞活化，同时促炎细胞因子（即 PaIL-1β 和 PaTNF-α）表达增加。总的来说，PaLL 样的这些免疫调节活性抑制了 V 的增殖。鳗鲡素靶向体内组织，可能有助于提高受感染鱼的存活率。总体而言，我们的结果表明 PaLL 样是先天免疫的关键组成部分，并提供针对细菌感染的保护作用。
Ladderlectin is a member of C-type lectins (CTLs) in teleost fish and involved in innate immune defense. In this study, ayu (Plecoglossus altivelis)ladderlecin-like(PaLL-like) sequence was cloned, which encodes a polypeptide of 172 amino acids that includes a signal peptide and characteristic C-type lectin-like domains (CTLDs). Phylogenetically,PaLL-like was most closely related to its teleost counterpart from shishamo smelt (Spirinchus lanceolatus).Expression analysis revealed a ubiquitous expression profile, with highest expression detected in liver and its expression was up-regulated followingVibiro anguillaruminfection. Similar to canonical CTLs,PaLL-like exhibited carbohydrate-binidng capacities to a wide range of well-defined mono-/di-saccharides and likely conferPaLL-like the ability to agglutinate all tested bacterial, including three Gram-positive species (i.e.,Listeria monocytogenes,Staphylococcus aureusandStreptococcus iniae) and eight Gram-negative species (i.e.,Edwardsiella tarda,Aeromonas(A.)hydrophila,Escherichia coli,Vibrio(V.)harveyi,V. anguillarum,V. parahemolyticus,A. versoniandV. vulnificus), in a calcium-dependent manner. Further functional studies revealed thatPaLL-like displayed immunomodulatory activities leading to enhanced bactericidal activity of serum, pathogen opsonization and macrophage activation with increased expression of pro-inflammatory cytokines (i.e.,PaIL-1βandPaTNF-α). Collectively, these immunomodulatory activities ofPaLL-like suppressed proliferations ofV. anguillarumin targeted tissuedin vivoand likely contributed to the increased survival rate of infected-fish. Overall, our results demonstratedPaLL-like is a critical component of innate immunity and provides protective effects against bacterial infection.