IgA and IgG against Mycobacterium tuberculosis Rv2031 discriminate between pulmonary tuberculosis patients, Mycobacterium tuberculosis-infected and non-infected individuals.
IgA and IgG against Mycobacterium tuberculosis Rv2031 discriminate between pulmonary tuberculosis patients, Mycobacterium tuberculosis-infected and non-infected individuals.
复制标题
针对结核分枝杆菌RV2031的IgA和IgG区分肺结核患者，结核分枝杆菌感染和未感染的个体。
DOI：
10.1371/journal.pone.0190989
复制
发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Ottenhoff THM
中科院分区：
文献类型：
--
作者：
Abebe F;Belay M;Legesse M;K L M C F;Ottenhoff THM
作为研究结核病 (TB) 保护性和诊断性免疫标志物重大项目的一部分，我们测量了流行环境中 149 名肺结核患者 (PTBP)、148 名家庭接触者 (HHC) 和 68 名社区对照 (CC) 的队列对结核分枝杆菌 (Mtb) 抗原（LAM、Rv2031 和 HBHA）的抗体同种型反应。使用 ELISA 测量基线以及入组后 6 个月和 12 个月时队列血清中的 IgA、IgG 和 IgM 水平。结果显示，三个队列中对不同抗原的 IgA、IgG 和 IgM 反应存在显着差异。基线时，针对 RV2031 和 LAM 的 IgM 水平在队列之间没有变化，但 PTB 患者中针对 Rv2031 的 IgA 和 IgG 水平显着高于 HHC 和 CC，其次是 HHC，CC 中最低。在患者中，化疗前后抗体反应存在显着差异。化疗后抗HBHA的IgA和IgG以及抗Rv2031的IgA水平显着下降并保持在低水平，而抗LAM的IgA和IgG水平显着升高并保持在高水平。然而，针对 Rv2031 和 LAM 的 IgM 水平在 6 个月时有所增加，但在 12 个月时再次下降。化疗前后针对 HBHA 的 IgM 没有表现出任何显着变化。同样，随着时间的推移，HHC 中的抗体反应也存在显着变化。我们的结果表明，在三个队列中，对不同抗原的 IgA、IgG 和 IgM 反应存在显着差异，这意味着并非所有抗体同种型反应都是临床结核病的标志物。此外，当前和之前的研究一致表明，针对 Rv2031 的 IgA 和 IgG 能够区分临床疾病、Mtb 感染者和未感染者。
As part of a major project to investigate protective and diagnostic immune markers against tuberculosis (TB), we measured antibody isotype responses to Mycobacterium tuberculosis (Mtb) antigens (LAM, Rv2031, and HBHA) in cohorts of 149 pulmonary tuberculosis patients (PTBP), 148 household contacts (HHCs), and 68 community controls (CCs) in an endemic setting. ELISA was used to measure levels of IgA, IgG, and IgM from sera of cohorts at baseline, and at 6 and 12 months from entry. The results show that there were significant differences in IgA, IgG, and IgM responses to the different antigens and in the three cohorts. At baseline, the level of IgM against RV2031 and LAM did not vary between cohorts, but the levels of IgA and IgG against Rv2031 were significantly higher in PTB patients than HHCs and CCs, followed by HHCs, and the lowest in CCs. In patients, there was a significant variation in antibody responses before and after chemotherapy. The levels of IgA and IgG against HBHA, and IgA against Rv2031 decreased significantly and remained low, while IgA and IgG against LAM increased significantly and remained high following chemotherapy. However, the levels of IgM against Rv2031 and LAM increased at 6 months but decreased again at 12 months. IgM against HBHA did not show any significant variation before and after chemotherapy. Similarly, there were also significant variations in antibody responses in HHCs over time. Our results show that there are significant variations in IgA, IgG and IgM responses to the different antigens and in the three cohorts, implying that not all antibody isotype responses are markers of clinical TB. In addition, the current and previous studies consistently show that IgA and IgG against Rv2031 discriminate between clinical disease, Mtb-infected and non-infected individuals.
登录
查看更多内容
DOI：
10.4049/jimmunol.1601199
发表时间：
2017-03-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Athman JJ;Sande OJ;Groft SG;Reba SM;Nagy N;Wearsch PA;Richardson ET;Rojas R;Boom WH;Shukla S;Harding CV
通讯作者：
Harding CV
影响因子：
3.7
作者：
Chegou NN;Essone PN;Loxton AG;Stanley K;Black GF;van der Spuy GD;van Helden PD;Franken KL;Parida SK;Klein MR;Kaufmann SH;Ottenhoff TH;Walzl G
通讯作者：
Walzl G
DOI：
10.1164/ajrccm.161.5.9908125
发表时间：
2000-05-01
影响因子：
24.7
作者：
Chan, ED;Reves, R;Hahn, WE
通讯作者：
Hahn, WE
影响因子：
3.1
作者：
CHAN, J;FAN, X;BLOOM, BR
通讯作者：
BLOOM, BR
DOI：
10.1073/pnas.1221708110
发表时间：
2013-05-28
影响因子：
11.1
作者：
Blattes, Emilyne;Vercellone, Alain;Puzo, Germain
通讯作者：
Puzo, Germain